Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 02, 2021

Anti-BubR1 antibody [EPR12259(2)] (ab183496)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12259(2)] to BubR1
Suitable for: WB, IHC-P, Flow Cyt, IP
Reacts with: Human

Product name

Anti-BubR1 antibody [EPR12259(2)]
See all BubR1 primary antibodies
Description

Rabbit monoclonal [EPR12259(2)] to BubR1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- HeLa, HepG2 and Jurkat lysates, Human spleen and Human ovarian carcinoma tissue, HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR12259(2)
Isotype

IgG
Research areas

Western blot - Anti-BubR1 antibody [EPR12259(2)] (ab183496)

Anti-BubR1 antibody [EPR12259(2)] (ab183496) at 1/20000 dilution + HeLa lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 120 kDa
Western blot - Anti-BubR1 antibody [EPR12259(2)] (ab183496)

All lanes : Anti-BubR1 antibody [EPR12259(2)] (ab183496) at 1/100000 dilution

Lane 1 : HepG2 lysate
Lane 2 : Jurkat lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 120 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BubR1 antibody [EPR12259(2)] (ab183496)

Immunohistochemical analysis of paraffin-embedded Human spleen labeling BubR1 with ab183496 at 1/100 dilution and HRP conjugated Rabbit IgG. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BubR1 antibody [EPR12259(2)] (ab183496)

Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma labeling BubR1 with ab183496 at 1/100 dilution and HRP conjugated Rabbit IgG. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Flow Cytometry - Anti-BubR1 antibody [EPR12259(2)] (ab183496)

Flow cytometric analysis of HeLa cells fixed in 2% paraformaldehyde labeling BubR1 with ab183496 at 1/100 dilution and Goat anti rabbit IgG (FITC). Rabbit monoclonal IgG was used as an isotype control.
Immunoprecipitation - Anti-BubR1 antibody [EPR12259(2)] (ab183496)

Immunoprecipitation of HepG2 cells labeling BubR1 with ab183496 at 1/50 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000.
Anti-BubR1 antibody [EPR12259(2)] (ab183496)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com