Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BubR1 antibody [EPR22544-48] - BSA and Azide free (ab255249)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22544-48] to BubR1 - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-BubR1 antibody [EPR22544-48] - BSA and Azide free
    See all BubR1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22544-48] to BubR1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
    Unsuitable for: Flow Cyt,IHC-Fr or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human breast cancer and testis tissue; Mouse and rat testis tissue. ICC/IF: SK-OV-3 and HeLa cells.

  • General notes

    Ab255249 is the carrier-free version of ab254326. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab255249 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BubR1 antibody [EPR22544-48] - BSA and Azide free (ab255249)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BubR1 antibody [EPR22544-48] - BSA and Azide free (ab255249)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling BubR1 with ab254326 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat testis (PMID: 20411023). The section was incubated with ab254326 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254326).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BubR1 antibody [EPR22544-48] - BSA and Azide free (ab255249)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BubR1 antibody [EPR22544-48] - BSA and Azide free (ab255249)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling BUB1B with ab254326 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse testis (PMID: 20411023). The section was incubated with ab254326 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

     

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254326).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BubR1 antibody [EPR22544-48] - BSA and Azide free (ab255249)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BubR1 antibody [EPR22544-48] - BSA and Azide free (ab255249)

    Immunohistochemical analysis of paraffin-embedded human testis tissue labeling BUB1B with ab254326 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human testis (PMID: 20411023). The section was incubated with ab254326 for 30 mins at room temperature. Counterstained with Hematoxylin.

     

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254326).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BubR1 antibody [EPR22544-48] - BSA and Azide free (ab255249)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BubR1 antibody [EPR22544-48] - BSA and Azide free (ab255249)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling BUB1B with ab254326 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human breast cancer. The section was incubated with ab254326 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

     

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254326).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-BubR1 antibody [EPR22544-48] - BSA and Azide free (ab255249)
    Immunocytochemistry/ Immunofluorescence - Anti-BubR1 antibody [EPR22544-48] - BSA and Azide free (ab255249)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-OV-3 (human ovarian cancer epithelial cell) cells labeling BubR1 with ab254326 at 1/50 dilution, followed by a AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution (Green). Confocal image showing specific staining on centromeres of SK-OV-3 cell line in M phase. The nuclear counterstain is DAPI (Blue). Tubulin was stained using Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889).


    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254326).
  • Immunocytochemistry/ Immunofluorescence - Anti-BubR1 antibody [EPR22544-48] - BSA and Azide free (ab255249)
    Immunocytochemistry/ Immunofluorescence - Anti-BubR1 antibody [EPR22544-48] - BSA and Azide free (ab255249)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling BubR1 with ab254326 at 1/50 dilution, followed by a AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution (Green).Confocal image showing cytoplasmic staining in HeLa cell line. The nuclear counterstain is DAPI (Blue). Tubulin was stained using Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution.


    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254326).
  • Immunocytochemistry/ Immunofluorescence - Anti-BubR1 antibody [EPR22544-48] - BSA and Azide free (ab255249)
    Immunocytochemistry/ Immunofluorescence - Anti-BubR1 antibody [EPR22544-48] - BSA and Azide free (ab255249)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling BubR1 with ab254326 at 1/50 dilution, followed by a AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution (Green).Confocal image showing specific staining on centromeres of HeLa cell line in M phase. The nuclear counterstain is DAPI (Blue). Tubulin was stained using Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution.


    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254326).
  • Anti-BubR1 antibody [EPR22544-48] - BSA and Azide free (ab255249)
    Anti-BubR1 antibody [EPR22544-48] - BSA and Azide free (ab255249)

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