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Anti-BubR1 antibody [EPR12259(2)] - BSA and Azide free (ab250655)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12259(2)] to BubR1 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IHC-P, IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-BubR1 antibody [EPR12259(2)] - BSA and Azide free
    See all BubR1 primary antibodies

  • Description

    Rabbit monoclonal [EPR12259(2)] to BubR1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), IHC-P, IP, WBmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250655 is the carrier-free version of ab183496.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-BubR1 antibody [EPR12259(2)] - BSA and Azide free (ab250655)
    Western blot - Anti-BubR1 antibody [EPR12259(2)] - BSA and Azide free (ab250655)
    Anti-BubR1 antibody [EPR12259(2)] (ab183496) at 1/20000 dilution + HeLa lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 120 kDa



    This data was developed using ab183496, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-BubR1 antibody [EPR12259(2)] - BSA and Azide free (ab250655)
    Western blot - Anti-BubR1 antibody [EPR12259(2)] - BSA and Azide free (ab250655)
    All lanes : Anti-BubR1 antibody [EPR12259(2)] (ab183496) at 1/100000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 120 kDa



    This data was developed using ab183496, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BubR1 antibody [EPR12259(2)] - BSA and Azide free (ab250655)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BubR1 antibody [EPR12259(2)] - BSA and Azide free (ab250655)
    This data was developed using ab183496, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human spleen labeling BubR1 with ab183496 at 1/100 dilution and HRP conjugated Rabbit IgG. Counterstained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BubR1 antibody [EPR12259(2)] - BSA and Azide free (ab250655)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BubR1 antibody [EPR12259(2)] - BSA and Azide free (ab250655)
    This data was developed using ab183496, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma labeling BubR1 with ab183496 at 1/100 dilution and HRP conjugated Rabbit IgG. Counterstained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-BubR1 antibody [EPR12259(2)] - BSA and Azide free (ab250655)
    Flow Cytometry - Anti-BubR1 antibody [EPR12259(2)] - BSA and Azide free (ab250655)
    This data was developed using ab183496, the same antibody clone in a different buffer formulation.Flow cytometric analysis of HeLa cells fixed in 2% paraformaldehyde labeling BubR1 with ab183496 at 1/100 dilution and Goat anti rabbit IgG (FITC). Rabbit monoclonal IgG was used as an isotype control.
  • Immunoprecipitation - Anti-BubR1 antibody [EPR12259(2)] - BSA and Azide free (ab250655)
    Immunoprecipitation - Anti-BubR1 antibody [EPR12259(2)] - BSA and Azide free (ab250655)
    This data was developed using ab183496, the same antibody clone in a different buffer formulation.Immunoprecipitation of HepG2 cells labeling BubR1 with ab183496 at 1/50 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000.
  • Anti-BubR1 antibody [EPR12259(2)] - BSA and Azide free (ab250655)
    Anti-BubR1 antibody [EPR12259(2)] - BSA and Azide free (ab250655)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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