Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IgA antibody [EPR5367-76] - BSA and Azide free (ab214003)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5367-76] to IgA - BSA and Azide free
  • Suitable for: IHC-Fr, IHC-P, ELISA, WB, ICC/IF, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-IgA antibody [EPR5367-76] - BSA and Azide free
    See all IgA primary antibodies

  • Description

    Rabbit monoclonal [EPR5367-76] to IgA - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Fr, IHC-P, ELISA, WB, ICC/IF, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Full length native protein (purified) corresponding to Human IgA.

  • Positive control

    • Human tonsil, Human plasma, and Human spleen lysates; Human colon tissue, Human tonsil tissue.

  • General notes

    ab214003 is the carrier-free version of ab124716 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab214003 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.20
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR5367-76

  • Isotype

    IgG

  • Research areas

Images

  • Immunoprecipitation - Anti-IgA antibody [EPR5367-76] - BSA and Azide free (ab214003)
    Immunoprecipitation - Anti-IgA antibody [EPR5367-76] - BSA and Azide free (ab214003)

    Capture ab 1:60 dilution (1μg in 1mg lysate). Primary ab for WB ab124716 at 1:1000 dilution (0.18μg/ml). Secondary ab Anti-Rabbit IgG (HRP), specific to the non5% NFDM/TBST -reduced form of IgG. Secondary ab concentration 1:1500 dilution. Blocking buffer and concentration 5% NFDM/TBST. Diluting buffer and concentration 5% NFDM/TBST Lane 1 (+) Human plasma Lane 2 (-) Rabbit monoclonal IgG (ab172730) instead of ab124716 in Human plasma

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124716).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA antibody [EPR5367-76] - BSA and Azide free (ab214003)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA antibody [EPR5367-76] - BSA and Azide free (ab214003)

    ab124716, at 1/100, staining IgA in paraffin embedded Human colon tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124716).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA antibody [EPR5367-76] - BSA and Azide free (ab214003)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA antibody [EPR5367-76] - BSA and Azide free (ab214003)

    ab124716, at 1/100, staining IgA in paraffin embedded Human tonsil tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124716).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-IgA antibody [EPR5367-76] - BSA and Azide free (ab214003)
    Immunohistochemistry (Frozen sections) - Anti-IgA antibody [EPR5367-76] - BSA and Azide free (ab214003)

    Clone EPR5367-76 (ab214003) has been successfully conjugated by Abcam. This image was generated using Anti-IgA antibody [EPR5367-76] (Alexa Fluor® 488). Please refer to ab223410 for protocol details.

    IHC image of IgA staining in a section of frozen normal human tonsil*.

    The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature.  The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab223410 at 1/5000 dilution (shown in green) and counterstained using ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • Anti-IgA antibody [EPR5367-76] - BSA and Azide free (ab214003)
    Anti-IgA antibody [EPR5367-76] - BSA and Azide free (ab214003)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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