Astana Biomed Group, an authorized Abcam distributor in Central Asia

Biotin Anti-IgA antibody [RM128] (ab224184)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Biotin Rabbit monoclonal [RM128] to IgA
  • Suitable for: ELISA, IHC-P, Sandwich ELISA
  • Reacts with: Human
  • Conjugation: Biotin

Overview

  • Product name

    Biotin Anti-IgA antibody [RM128]
    See all IgA primary antibodies

  • Description

    Biotin Rabbit monoclonal [RM128] to IgA

  • Host species

    Rabbit

  • Conjugation

    Biotin

  • Tested applications

    Suitable for: ELISA, IHC-P, Sandwich ELISAmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Full length protein corresponding to Human IgA.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), PBS, 1% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    ab224184 was purified from an animal origin–free culture supernatant.

  • Clonality

    Monoclonal

  • Clone number

    RM128

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-IgA antibody [RM128] (ab224184)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-IgA antibody [RM128] (ab224184)

    Paraffin-embedded human lymphoid tissue stained for IgA using ab224184 at 1 μg/mL in immunohistochemical analysis.

  • Sandwich ELISA - Biotin Anti-IgA antibody [RM128] (ab224184)
    Sandwich ELISA - Biotin Anti-IgA antibody [RM128] (ab224184)

    Sandwich ELISA using ab224184 as the capture antibody (100 ng/well), and Biotinylated anti-human light chains (κ+ λ) antibody ab193185 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.

  • Sandwich ELISA - Biotin Anti-IgA antibody [RM128] (ab224184)
    Sandwich ELISA - Biotin Anti-IgA antibody [RM128] (ab224184)

    Sandwich ELISA using ab224184 as the capture antibody (100 ng/well), and Biotinylated anti-human light chains (κ+ λ) antibody ab193185 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.

  • ELISA - Biotin Anti-IgA antibody [RM128] (ab224184)
    ELISA - Biotin Anti-IgA antibody [RM128] (ab224184)

    ELISA of human immunoglobulins shows ab224184 reacts to both Human IgA1 & IgA2. No cross reactivity with Human IgG, IgM, IgD, or IgE. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab224184 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

  • ELISA - Biotin Anti-IgA antibody [RM128] (ab224184)
    ELISA - Biotin Anti-IgA antibody [RM128] (ab224184)

    A titer ELISA using ab224184. The plate was coated with different amounts of human IgA. A serial dilution of ab224184 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

  • Biotin Anti-IgA antibody [RM128] (ab224184)
    Biotin Anti-IgA antibody [RM128] (ab224184)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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