Biotin Anti-IgA antibody [RM128] (ab224184)
![Biotin Anti-IgA antibody [RM128] (ab224184)](https://www.abcam.com/ps/products/224/ab224184/Images/ab224184-284647-IgAIHC.jpg "Biotin Anti-IgA antibody [RM128] (ab224184)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Biotin Rabbit monoclonal [RM128] to IgA
- Suitable for: ELISA, IHC-P, Sandwich ELISA
- Reacts with: Human
- Conjugation: Biotin
Overview
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Product name
Biotin Anti-IgA antibody [RM128]
See all IgA primary antibodies -
Description
Biotin Rabbit monoclonal [RM128] to IgA -
Host species
Rabbit -
Conjugation
Biotin -
Tested applications
Suitable for: ELISA, IHC-P, Sandwich ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein corresponding to Human IgA.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab224184 was purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM128 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-IgA antibody [RM128] (ab224184)
Paraffin-embedded human lymphoid tissue stained for IgA using ab224184 at 1 μg/mL in immunohistochemical analysis.
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![Sandwich ELISA - Biotin Anti-IgA antibody [RM128] (ab224184)](https://www.abcam.com/ps/products/224/ab224184/Images/ab224184-284648-IgAELISA1.jpg)
Sandwich ELISA - Biotin Anti-IgA antibody [RM128] (ab224184)Sandwich ELISA using ab224184 as the capture antibody (100 ng/well), and Biotinylated anti-human light chains (κ+ λ) antibody ab193185 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
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![Sandwich ELISA - Biotin Anti-IgA antibody [RM128] (ab224184)](https://www.abcam.com/ps/products/224/ab224184/Images/ab224184-284649-IgAELISA2.jpg)
Sandwich ELISA - Biotin Anti-IgA antibody [RM128] (ab224184)Sandwich ELISA using ab224184 as the capture antibody (100 ng/well), and Biotinylated anti-human light chains (κ+ λ) antibody ab193185 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
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![ELISA - Biotin Anti-IgA antibody [RM128] (ab224184)](https://www.abcam.com/ps/products/224/ab224184/Images/ab224184-284650-ab224184284650IgAELISA3.jpg)
ELISA - Biotin Anti-IgA antibody [RM128] (ab224184)ELISA of human immunoglobulins shows ab224184 reacts to both Human IgA1 & IgA2. No cross reactivity with Human IgG, IgM, IgD, or IgE. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab224184 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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![ELISA - Biotin Anti-IgA antibody [RM128] (ab224184)](https://www.abcam.com/ps/products/224/ab224184/Images/ab224184-284651-ab224184284651IgAELISA4.jpg)
ELISA - Biotin Anti-IgA antibody [RM128] (ab224184)A titer ELISA using ab224184. The plate was coated with different amounts of human IgA. A serial dilution of ab224184 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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![Biotin Anti-IgA antibody [RM128] (ab224184)](https://www.abcam.com/ps/products/224/ab224184/Images/ab224184-6-benefits-of-recombinant-antibodies.png)
Biotin Anti-IgA antibody [RM128] (ab224184)
