Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TROP2 antibody [SP293] - C-terminal (ab227689)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP293] to TROP2 - C-terminal
  • Suitable for: WB, Flow Cyt, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-TROP2 antibody [SP293] - C-terminal
    See all TROP2 primary antibodies

  • Description

    Rabbit monoclonal [SP293] to TROP2 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Mouse
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: SK-BR-3, bEnd.3, 4T1, LLC1, and B16F10 whole cell lysates; Flow Cyt: SK-BR-3 cells; IHC-P: Human tonsil, prostate, esophagus, bladder, skin, skin squamous cell carcinoma, lung squamous cell carcinoma, bladder transitional cell carcinoma, prostate adenocarcinoma and ovarian adenocarcinoma tissues. Mouse skin tissue.

  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...

  • Purity

    Protein A/G purified

  • Purification notes

    Purified from TCS by protein A/G.

  • Clonality

    Monoclonal

  • Clone number

    SP293

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-TROP2 antibody [SP293] - C-terminal (ab227689)
    Western blot - Anti-TROP2 antibody [SP293] - C-terminal (ab227689)
    All lanes : Anti-TROP2 antibody [SP293] - C-terminal (ab227689) at 1/100 dilution

    Lane 1 : bEnd.3 (mouse brain endothelioma) whole cell lysate
    Lane 2 : 4T1 (mouse mammary gland carcinoma epithelial cell) whole cell lysate
    Lane 3 : LLC1 (mouse lung carcinoma) whole cell lysate
    Lane 4 : B16F10 (Mouse skin melanoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 36 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    Blocking/Diluting buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (ab227689)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (ab227689)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse skin tissue labelling TROP2 with ab227689 at 1/50 dilution.  Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 for 20 minutes. The section was incubated with ab227689 for 10 mins at room temperature. The Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used. Membranous staining on the mouse skin. Performed on a Leica Biosystems BOND® RX instrument. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Flow Cytometry - Anti-TROP2 antibody [SP293] - C-terminal (ab227689)
    Flow Cytometry - Anti-TROP2 antibody [SP293] - C-terminal (ab227689)

    Flow Cytometry analysis of SK-BR-3 (human mammary gland adenocarcinoma cell line) cells, labeling TROP2 with ab227689 at 1/100 dilution (green) compared to a Rabbit IgG negative control (blue).

  • Western blot - Anti-TROP2 antibody [SP293] - C-terminal (ab227689)
    Western blot - Anti-TROP2 antibody [SP293] - C-terminal (ab227689)
    Anti-TROP2 antibody [SP293] - C-terminal (ab227689) at 1/100 dilution + SK-BR-3 (human mammary gland adenocarcinoma cell line) cell lysate

    Predicted band size: 36 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (ab227689)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (ab227689)

    Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (ab227689)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (ab227689)

    Formalin-fixed, paraffin-embedded human esophagus tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (ab227689)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (ab227689)

    Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (ab227689)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (ab227689)

    Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (ab227689)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (ab227689)

    Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (ab227689)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (ab227689)

    Formalin-fixed, paraffin-embedded human prostate tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (ab227689)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (ab227689)

    Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (ab227689)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (ab227689)

    Formalin-fixed, paraffin-embedded human bladder tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (ab227689)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (ab227689)

    Formalin-fixed, paraffin-embedded human skin tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (ab227689)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (ab227689)

    Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (ab227689)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (ab227689)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

  • Anti-TROP2 antibody [SP293] - C-terminal (ab227689)
    Anti-TROP2 antibody [SP293] - C-terminal (ab227689)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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