Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸ 10 µl 113 913 ₸

Order now and get it on Wednesday March 03, 2021

Anti-CD39 antibody [EPR20461] (ab223843)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20461] to CD39
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-CD39 antibody [EPR20461]
See all CD39 primary antibodies
Description

Rabbit monoclonal [EPR20461] to CD39
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human fetal heart, fetal spleen, tonsil and fetal kidney lysates. IHC-P: Human pancreas, colon carcinoma and tonsil tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20461
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] (ab223843)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling CD39 with ab223843 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on endothelial cells of human pancreas (PMID: 18567823; PMID: 19308674). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] (ab223843)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling CD39 with ab223843 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on stromal cells of human colon carcinoma (PMID: 25403716). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] (ab223843)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD39 with ab223843 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining on human tonsil (PMID: 27532024). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-CD39 antibody [EPR20461] (ab223843)

All lanes : Anti-CD39 antibody [EPR20461] (ab223843) at 1/1000 dilution

Lane 1 : Human fetal heart lysate at 20 µg
Lane 2 : Human fetal spleen lysate at 20 µg
Lane 3 : Human tonsil lysate at 20 µg
Lane 4 : Human fetal kidney lysate at 10 µg

Secondary
Lanes 1-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 4 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 58 kDa
Observed band size: 78 kDa
why is the actual band size different from the predicted?

Exposure time : Lanes 1,4: 3 minutes; Lanes 2-3: 10 seconds.

Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-CD39 antibody [EPR20461] (ab223843)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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