Anti-CD163 antibody [OTI2G12] (ab156769)
![Anti-CD163 antibody [OTI2G12] (ab156769)](https://www.abcam.com/ps/products/156/ab156769/Images/ab156769-163856-anti-cd163-antibody-oti2g12-immunohistochemistry.jpg "Anti-CD163 antibody [OTI2G12] (ab156769)")
Key features and details
- Mouse monoclonal [OTI2G12] to CD163
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG1
Overview
-
Product name
Anti-CD163 antibody [OTI2G12]
See all CD163 primary antibodies -
Description
Mouse monoclonal [OTI2G12] to CD163 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF African green monkeyIHC-P HumanWB Human
-
Immunogen
Recombinant full length protein corresponding to Human CD163.
Database link: Q86VB7 -
Positive control
- WB: HEK-293T cell lysate transfected with pCMV6-ENTRY CD163 cDNA. IHC-P: Human liver tissue. ICC/IF: COS-7 cells transiently transfected with pCMV6-ENTRY CD163.
-
General notes
Dilute in PBS (pH7.3) before use.
The clone number has been updated from 2G12 to OTI2G12, both clone numbers name the same clone.
This product was changed from ascites to tissue culture supernatant on 6th September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, PBS, 1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
ab156769 was purified from cell culture supernatant by affinity chromatography. -
Clonality
Monoclonal -
Clone number
OTI2G12 -
Isotype
IgG1 -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [OTI2G12] (ab156769)
Paraffin-embedded human liver tissue stained for CD163 with ab156769 (1/150 dilution) in immunohistochemical analysis.
-
![Western blot - Anti-CD163 antibody [OTI2G12] (ab156769)](https://www.abcam.com/ps/products/156/ab156769/Images/ab156769-289705-anti-cd163-antibody-oti2g12-western-blot.jpg)
Western blot - Anti-CD163 antibody [OTI2G12] (ab156769)Sample: Human tissue lysate - whole (Skeletal muscle (Gastrocnemius).
Specification: Skeletal muscle (Gastrocnemius).
Blocking step: Milk as blocking agent for 1 hour(s) and 0 minute(s) Concentration: 5% Temperature: 25°C.ab156769 used at a 1/1000 dilution.
A Goat Anti-Mouse Alexa Fluor® 488 was used as the secondary at a 1/5000 dilution.
CON = Healthy patient known to express basal levels of macrophages.
POS = Patient of interest Non specific bands more faint than band of interest in POS. -
![Immunocytochemistry/ Immunofluorescence - Anti-CD163 antibody [OTI2G12] (ab156769)](https://www.abcam.com/ps/products/156/ab156769/Images/ab156769-291865-anti-cd163-antibody-oti2g12-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD163 antibody [OTI2G12] (ab156769) This image is courtesy of an anonymous Abreviewab156769 staining CD163 in Human skeletal muscle (Gastrocnemius) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methacarn and blocked with 10% serum for 20 minutes at 25°C. Samples were incubated with primary antibody (1/100) for 14 hours at 4°C. An Alexa Fluor® 555-conjugated Goat anti-mouse polyclonal was used as the secondary antibody (1/200).
-
![Immunocytochemistry/ Immunofluorescence - Anti-CD163 antibody [OTI2G12] (ab156769)](https://www.abcam.com/ps/products/156/ab156769/Images/ab156769-163857-anti-cd163-antibody-oti2g12-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD163 antibody [OTI2G12] (ab156769)Immunofluorescent analysis of COS-7 (african green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY CD163 labeling CD163 with ab156769 at 1/100 dilution.
-
![Western blot - Anti-CD163 antibody [OTI2G12] (ab156769)](https://www.abcam.com/ps/products/156/ab156769/Images/ab156769-163858-anti-cd163-antibody-oti2g12-western-blot.jpg)
Western blot - Anti-CD163 antibody [OTI2G12] (ab156769)All lanes : Anti-CD163 antibody [OTI2G12] (ab156769) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY CD163 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 125 kDaHEK-293T cell lysates were generated from transient transfection of the cDNA clone (RC212446)
