Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Wednesday March 10, 2021

Anti-CD163 antibody [EPR14336] (ab199427)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14336] to CD163
Suitable for: IHC-P, WB
Reacts with: Human

Product name

Anti-CD163 antibody [EPR14336]
See all CD163 primary antibodies
Description

Rabbit monoclonal [EPR14336] to CD163
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human spleen, fetal liver and placenta lysate. IHC-P: Human liver and thymus tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14336
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR14336] (ab199427)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling CD163 with ab199427 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Kupffer cells staining in liver tissue is observed. Counter stained with Hematoxylin.
Negative control: Used only secondary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-CD163 antibody [EPR14336] (ab199427)

All lanes : Anti-CD163 antibody [EPR14336] (ab199427) at 1/2000 dilution

Lane 1 : Human spleen lysate
Lane 2 : Human fetal liver lysate
Lane 3 : Human placenta lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 125 kDa
Observed band size: 150 kDa
why is the actual band size different from the predicted?

Exposure time: 30 seconds

Blocking/dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR14336] (ab199427)

Immunohistochemical analysis of paraffin-embedded Human thymus tissue labeling CD163 with ab199427 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining on human thymus tissue is observed. Counter stained with Hematoxylin.
Negative control: Used only secondary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-CD163 antibody [EPR14336] (ab199427)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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