Anti-Podoplanin / gp36 antibody [PMab-1] (ab256559)
![Anti-Podoplanin / gp36 antibody [PMab-1] (ab256559)](https://www.abcam.com/ps/products/256/ab256559/Images/ab256559-392171-anti-podoplaningp36-antibody-pmab1-immunohistochemistry-mouse-lung.jpg "Anti-Podoplanin / gp36 antibody [PMab-1] (ab256559)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [PMab-1] to Podoplanin / gp36
- Suitable for: IHC-P, IHC-Fr, WB
- Reacts with: Mouse
Overview
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Product name
Anti-Podoplanin / gp36 antibody [PMab-1]
See all Podoplanin / gp36 primary antibodies -
Description
Rat monoclonal [PMab-1] to Podoplanin / gp36 -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species IHC-Fr MouseIHC-P MouseWB Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse lung tissue lysate. IHC-P: Mouse kidney and lung tissue. IHC-Fr: Mouse kidney and lung tissue.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Ion Exchange Chromatography -
Clonality
Monoclonal -
Clone number
PMab-1 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin / gp36 antibody [PMab-1] (ab256559)
Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Podoplanin / gp36 with ab256559 at 1/1000 dilution followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on mouse lung is observed. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Western blot - Anti-Podoplanin / gp36 antibody [PMab-1] (ab256559)](https://www.abcam.com/ps/products/256/ab256559/Images/ab256559-392172-anti-podoplaningp36-antibody-pmab1-western-blot-mouse-lung-tissue-lysate-20-ug.jpg)
Western blot - Anti-Podoplanin / gp36 antibody [PMab-1] (ab256559)Anti-Podoplanin / gp36 antibody [PMab-1] (ab256559) at 1/1000 dilution + mouse lung tissue lysate at 20 µg
Secondary
Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution
Predicted band size: 16 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time 26 secs.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin / gp36 antibody [PMab-1] (ab256559)](https://www.abcam.com/ps/products/256/ab256559/Images/ab256559-392170-anti-podoplaningp36-antibody-pmab1-immunohistochemistry-mouse-kidney1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin / gp36 antibody [PMab-1] (ab256559)Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Podoplanin / gp36 with ab256559 at 1/1000 dilution followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on the podocytes in the mouse kidney is observed. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Immunohistochemistry (Frozen sections) - Anti-Podoplanin / gp36 antibody [PMab-1] (ab256559)](https://www.abcam.com/ps/products/256/ab256559/Images/ab256559-392169-anti-podoplaningp36-antibody-pmab1-immunohistochemistry-frozen-mouse-lung.jpg)
Immunohistochemistry (Frozen sections) - Anti-Podoplanin / gp36 antibody [PMab-1] (ab256559)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse lung tissue labeling Podoplanin / gp36 with ab256559 at 1/500 dilution followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse lung is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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![Immunohistochemistry (Frozen sections) - Anti-Podoplanin / gp36 antibody [PMab-1] (ab256559)](https://www.abcam.com/ps/products/256/ab256559/Images/ab256559-392168-anti-podoplaningp36-antibody-pmab1-immunohistochemistry-frozen-mouse-kidney.jpg)
Immunohistochemistry (Frozen sections) - Anti-Podoplanin / gp36 antibody [PMab-1] (ab256559)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney tissue labeling Podoplanin / gp36 with ab256559 at 1/500 dilution followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse kidney is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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![Anti-Podoplanin / gp36 antibody [PMab-1] (ab256559)](https://www.abcam.com/ps/products/256/ab256559/Images/ab256559-6-benefits-of-recombinant-antibodies.png)
Anti-Podoplanin / gp36 antibody [PMab-1] (ab256559)