Biotin Anti-CD163 antibody [EPR14643-36] - C-terminal (ab218295)
![Biotin Anti-CD163 antibody [EPR14643-36] - C-terminal (ab218295)](https://www.abcam.com/ps/products/218/ab218295/Images/ab218295-279590-anti-cd163-antibody-epr14643-36-c-terminal-biotin-immunohistochemistry.jpg "Biotin Anti-CD163 antibody [EPR14643-36] - C-terminal (ab218295)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Biotin Rabbit monoclonal [EPR14643-36] to CD163 - C-terminal
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: Biotin
Overview
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Product name
Biotin Anti-CD163 antibody [EPR14643-36] - C-terminal
See all CD163 primary antibodies -
Description
Biotin Rabbit monoclonal [EPR14643-36] to CD163 - C-terminal -
Host species
Rabbit -
Conjugation
Biotin -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Recombinant fragment within Human CD163 aa 1000 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q86VB7 -
Positive control
- IHC-P: normal human liver tissue sections
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 30% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14643-36 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-CD163 antibody [EPR14643-36] - C-terminal (ab218295)
IHC image of CD163 staining in a section of formalin-fixed paraffin-embedded normal human liver*.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Biocare Medical NxGen pressure cooker using retrieval settings of 110oC for 8 minutes. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C with ab218295 at 1/100 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Biotin Anti-CD163 antibody [EPR14643-36] - C-terminal (ab218295)](https://www.abcam.com/ps/products/218/ab218295/Images/ab218295-2-benefits-of-recombinant-antibodies.png)
Biotin Anti-CD163 antibody [EPR14643-36] - C-terminal (ab218295)
