Anti-BFL-1/GRS antibody [SP435] - BSA and Azide free (ab253247)
![Anti-BFL-1/GRS antibody [SP435] - BSA and Azide free (ab253247)](https://www.abcam.com/ps/products/253/ab253247/Images/ab253247-349550-anti-bcl2a1-grs-antibody-sp435-immunohistochemistry-b-cell-lymphoma-human-.jpg "Anti-BFL-1/GRS antibody [SP435] - BSA and Azide free (ab253247)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP435] to BFL-1/GRS - BSA and Azide free
- Suitable for: IHC-P
- Reacts with: Human
Overview
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Product name
Anti-BFL-1/GRS antibody [SP435] - BSA and Azide free
See all BFL-1/GRS primary antibodies -
Description
Rabbit monoclonal [SP435] to BFL-1/GRS - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Recombinant fragment within Human BFL-1/GRS aa 1-150. The exact sequence is proprietary.
Database link: Q16548 -
Positive control
- IHC-P: Human B-cell lymphoma tissue.
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General notes
FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Ab253247 is the carrier-free version of ab246319. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab253247 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
SP435 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BFL-1/GRS antibody [SP435] - BSA and Azide free (ab253247)
IHC image of ab246319 staining BFL-1/GRS in a section of formalin-fixed paraffin-embedded human B-cell lymphoma* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH9, epitope retrieval solution 2) for 30mins. The section was then incubated with ab246319, 1/117 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab246319).
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![Anti-BFL-1/GRS antibody [SP435] - BSA and Azide free (ab253247)](https://www.abcam.com/ps/products/253/ab253247/Images/ab253247-2-benefits-of-recombinant-antibodies.png)
Anti-BFL-1/GRS antibody [SP435] - BSA and Azide free (ab253247)
