Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Thursday March 04, 2021

Anti-JNK1 antibody [EPR18841-99] (ab213521)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18841-99] to JNK1
Suitable for: WB, IP
Reacts with: Mouse, Rat, Human, Recombinant fragment

Product name

Anti-JNK1 antibody [EPR18841-99]
See all JNK1 primary antibodies
Description

Rabbit monoclonal [EPR18841-99] to JNK1
Host species

Rabbit

Tested Applications & Species

Application	Species
IP	Human
WB	Mouse Rat Human Recombinant fragment

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human JNK1 full length recombinant protein; Human fetal brain, fetal heart and fetal kidney lysates; Jurkat, HeLa, K562, MCF7, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Mouse brain, kidney and spleen lysates; Rat brain, heart, kidney and spleen lysates. IP: HeLa whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18841-99
Isotype

IgG
Research areas

Western blot - Anti-JNK1 antibody [EPR18841-99] (ab213521)

All lanes : Anti-JNK1 antibody [EPR18841-99] (ab213521) at 1/1000 dilution

Lane 1 : Human JNK1 full length recombinant protein
Lane 2 : Human JNK2 full length recombinant protein
Lane 3 : Human JNK3 full length recombinant protein

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 48 kDa
Observed band size: 48 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1: 1 second; Lane 2 and 3: 3 minutes.

Human JNK1 full length recombinant protein contain aa1-427 with a His-Tag®. Human JNK2 full length recombinant protein contain aa1-424 with a His-Tag®. Human JNK3 full length recombinant protein contain aa1-464 with a His-Tag®.
Western blot - Anti-JNK1 antibody [EPR18841-99] (ab213521)

All lanes : Anti-JNK1 antibody [EPR18841-99] (ab213521) at 1/1000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 48 kDa
Observed band size: 46-54 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID: 11313405).
Western blot - Anti-JNK1 antibody [EPR18841-99] (ab213521)

All lanes : Anti-JNK1 antibody [EPR18841-99] (ab213521) at 1/1000 dilution

Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 48 kDa
Observed band size: 46-54 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID: 11313405).
Western blot - Anti-JNK1 antibody [EPR18841-99] (ab213521)

All lanes : Anti-JNK1 antibody [EPR18841-99] (ab213521) at 1/1000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Rat brain lysate
Lane 5 : Rat heart lysate
Lane 6 : Rat kidney lysate
Lane 7 : Rat spleen lysate
Lane 8 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 9 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 10 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 11 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 48 kDa
Observed band size: 46-54 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1,2,3,4,5,6 and 7: 3 minutes; Lane 8,9,10 and 11: 15 seconds.

The molecular weight observed is consistent with what has been described in the literature (PMID: 11313405).
Western blot - Anti-JNK1 antibody [EPR18841-99] (ab213521)

All lanes : Anti-JNK1 antibody [EPR18841-99] (ab213521) at 1/1000 dilution

Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate with Human JNK1 recombinant protein fragment (immunogen).

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 48 kDa
Observed band size: 46-54 kDa why is the actual band size different from the predicted?

Exposure time: 3 seconds
Immunoprecipitation - Anti-JNK1 antibody [EPR18841-99] (ab213521)

JNK1 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab213521 at 1/40 dilution.

Western blot was performed from the immunoprecipitate using ab213521 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Lane 1: HeLa whole cell lysate, 10ug (Input).

Lane 2: ab213521 IP in HeLa whole cell lysate.

Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab213521 in HeLa whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 10 seconds.
Anti-JNK1 antibody [EPR18841-99] (ab213521)

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