Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-JNK1 (phospho T183 + Y185) antibody [EPR20763] - BSA and Azide free (ab227061)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20763] to JNK1 (phospho T183 + Y185) - BSA and Azide free
  • Suitable for: WB, Dot blot, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-JNK1 (phospho T183 + Y185) antibody [EPR20763] - BSA and Azide free
    See all JNK1 primary antibodies

  • Description

    Rabbit monoclonal [EPR20763] to JNK1 (phospho T183 + Y185) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    Based on sequence homology this antibody cross reacts with JNK2 (phospho T183/Y185) and JNK3 (phospho T221/Y223).

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293 treated with 40 J/m² UV-C light, then cultivated in RPMI 1640 media containing 10% FBS for 30 minutes cell lysate.

  • General notes

    Ab227061 is the carrier-free version of ab215208. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab227061 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-JNK1 (phospho T183 + Y185) antibody [EPR20763] - BSA and Azide free (ab227061)
    Western blot - Anti-JNK1 (phospho T183 + Y185) antibody [EPR20763] - BSA and Azide free (ab227061)
    All lanes : Anti-JNK1 (phospho T183 + Y185) antibody [EPR20763] (ab215208)

    Lane 1 : Untreated HEK-293 (human epithelial cell line from embryonic kidney) cultivated in RPMI 1640 media containing 10% FBS for 30 minutes, whole cell lysate
    Lane 2 : HEK-293 treated with 40 J/m² UV-C light, then cultivated in RPMI 1640 media containing 10% FBS for 30 minutes, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 48 kDa
    Observed band size: 46, 54 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution: 5% NFDM/TBST.

    Phosphorylation of JNK at T183/Y185 can be induced by UV-C treatment (PMID: 15386344).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215208).

  • Immunoprecipitation - Anti-JNK1 (phospho T183 + Y185) antibody [EPR20763] - BSA and Azide free (ab227061)
    Immunoprecipitation - Anti-JNK1 (phospho T183 + Y185) antibody [EPR20763] - BSA and Azide free (ab227061)

    JNK1 (phospho T183 + Y185) was immunoprecipitated from 0.35mg of HEK-293 (human epithelial cell line from embryonic kidney) treated with 40 J/m² UV-C light, then cultivated in RPMI 1640 media containing 10% FBS for 30 minutes whole cell lysate with ab215208 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215208 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

    Lane 1: HEK-293 (human epithelial cell line from embryonic kidney) treated with 40 J/m² UV-C light, then cultivated in RPMI 1640 media containing 10% FBS for 30 minutes whole cell lysate 10 µg (Input). 

    Lane 2: ab215208 IP in HEK-293 treated with 40 J/m² UV-C light, then cultivated in RPMI 1640 media containing 10% FBS for 30 minutes whole cell lysate (+). 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab215208 in HEK-293 treated with 40 J/m² UV-C light, then cultivated in RPMI 1640 media containing 10% FBS for 30 minutes whole cell lysate (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215208).

  • Dot Blot - Anti-JNK1 (phospho T183 + Y185) antibody [EPR20763] - BSA and Azide free (ab227061)
    Dot Blot - Anti-JNK1 (phospho T183 + Y185) antibody [EPR20763] - BSA and Azide free (ab227061)

    Dot blot analysis of JNK1 (phospho T183+ Y185) labeled with ab215208 at 1/1000 dilution.

    Lane 1: JNK1 (phospho T183/Y185) peptide.
    Lane 2: JNK1 (phospho T183) peptide.
    Lane 3: JNK1 (phospho Y185) peptide.
    Lane 4: JNK1 non-phospho peptide.

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100,000 dilution was used as secondary antibody.

    Based on sequence homology this antibody also reacts with JNK2 (phospho T183/Y185) and JNK3 (phospho T221/Y223).

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215208).

  • Anti-JNK1 (phospho T183 + Y185) antibody [EPR20763] - BSA and Azide free (ab227061)
    Anti-JNK1 (phospho T183 + Y185) antibody [EPR20763] - BSA and Azide free (ab227061)

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