Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Wednesday March 10, 2021

Anti-JNK1 (phospho T183 + Y185) antibody [EPR20763] (ab215208)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20763] to JNK1 (phospho T183 + Y185)
Suitable for: Dot blot, IP, WB
Reacts with: Human

Product name

Anti-JNK1 (phospho T183 + Y185) antibody [EPR20763]
See all JNK1 primary antibodies
Description

Rabbit monoclonal [EPR20763] to JNK1 (phospho T183 + Y185)
Host species

Rabbit
Specificity

Based on sequence homology this antibody cross reacts with JNK2 (phospho T183/Y185) and JNK3 (phospho T221/Y223).
Tested Applications & Species

Application Species
IP
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HEK-293 treated with 40 J/m² UV-C light, then cultivated in RPMI 1640 media containing 10% FBS for 30 minutes and U-2 OS treated with 50 J/m² UV-C light, then cultivated in RPMI 1640 media containing 10% FBS for 2 hours cell lysate. Dot Blot: JNK1 (phospho T183/Y185) peptide. IP: HEK-293 treated with 40 J/m² UV-C light, then cultivated in RPMI 1640 media containing 10% FBS for 30 minutes cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20763
Isotype

IgG
Research areas

Western blot - Anti-JNK1 (phospho T183 + Y185) antibody [EPR20763] (ab215208)

All lanes : Anti-JNK1 (phospho T183 + Y185) antibody [EPR20763] (ab215208) at 1/1000 dilution

Lane 1 : Untreated HEK-293 (human epithelial cell line from embryonic kidney) cultivated in RPMI 1640 media containing 10% FBS for 30 minutes, whole cell lysate
Lane 2 : HEK-293 treated with 40 J/m² UV-C light, then cultivated in RPMI 1640 media containing 10% FBS for 30 minutes, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 48 kDa
Observed band size: 46, 54 kDa
why is the actual band size different from the predicted?

Exposure time: 1 minute

Blocking/Dilution: 5% NFDM/TBST.

Phosphorylation of JNK at T183/Y185 can be induced by UV-C treatment (PMID: 15386344).
Dot Blot - Anti-JNK1 (phospho T183 + Y185) antibody [EPR20763] (ab215208)

Dot blot analysis of JNK1 (phospho T183+ Y185) labeled with ab215208 at 1/1000 dilution.

Lane 1: JNK1 (phospho T183/Y185) peptide.
Lane 2: JNK1 (phospho T183) peptide.
Lane 3: JNK1 (phospho Y185) peptide.
Lane 4: JNK1 non-phospho peptide.

Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100,000 dilution was used as secondary antibody.

Based on sequence homology this antibody also reacts with JNK2 (phospho T183/Y185) and JNK3 (phospho T221/Y223).

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.
Immunoprecipitation - Anti-JNK1 (phospho T183 + Y185) antibody [EPR20763] (ab215208)

JNK1 (phospho T183 + Y185) was immunoprecipitated from 0.35mg of HEK-293 (human epithelial cell line from embryonic kidney) treated with 40 J/m² UV-C light, then cultivated in RPMI 1640 media containing 10% FBS for 30 minutes whole cell lysate with ab215208 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215208 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Lane 1: HEK-293 (human epithelial cell line from embryonic kidney) treated with 40 J/m² UV-C light, then cultivated in RPMI 1640 media containing 10% FBS for 30 minutes whole cell lysate 10 µg (Input).

Lane 2: ab215208 IP in HEK-293 treated with 40 J/m² UV-C light, then cultivated in RPMI 1640 media containing 10% FBS for 30 minutes whole cell lysate (+).

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab215208 in HEK-293 treated with 40 J/m² UV-C light, then cultivated in RPMI 1640 media containing 10% FBS for 30 minutes whole cell lysate (-).

Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 3 minutes.
Western blot - Anti-JNK1 (phospho T183 + Y185) antibody [EPR20763] (ab215208)

All lanes : Anti-JNK1 (phospho T183 + Y185) antibody [EPR20763] (ab215208) at 1/1000 dilution

Lane 1 : Untreated U-2 OS (human bone osteosarcoma epithelial cell line) cultivated in RPMI 1640 media containing 10% FBS for 2 hours, whole cell lysate
Lane 2 : U-2 OS treated with 50 J/m² UV-C light, then cultivated in RPMI 1640 media containing 10% FBS for 2 hours, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 48 kDa
Observed band size: 46, 54 kDa why is the actual band size different from the predicted?

Blocking/Dilution: 5% NFDM/TBST.

Phosphorylation of JNK at T183/Y185 can be induced by UV-C treatment (PMID: 15386344).
Anti-JNK1 (phospho T183 + Y185) antibody [EPR20763] (ab215208)

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