Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Ku70 antibody [EPR4027] - BSA and Azide free (ab239912)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4027] to Ku70 - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt, ICC/IF, IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Ku70 antibody [EPR4027] - BSA and Azide free
    See all Ku70 primary antibodies

  • Description

    Rabbit monoclonal [EPR4027] to Ku70 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A549, 293T, A431 , and HeLa lysates. IHC-P: human colon carcinoma, tonsil and testis tissue. ICC/IF: HeLa cells. Flow Cyt: HeLa cells. IP: HeLa cells.

  • General notes

    ab239912 is the carrier-free version of ab92450 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab239912 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunoprecipitation - Anti-Ku70 antibody [EPR4027] - BSA and Azide free (ab239912)
    Immunoprecipitation - Anti-Ku70 antibody [EPR4027] - BSA and Azide free (ab239912)

    ab92450 (purified ) at 1/20 dilution (0.5ug) immunoprecipitating Ku70 in HeLa whole cell lysate.

    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg.
    Lane 2 (+): ab92450 & HeLa whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab92450 in HeLa whole cell lysate.

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92450).

  • Flow Cytometry - Anti-Ku70 antibody [EPR4027] - BSA and Azide free (ab239912)
    Flow Cytometry - Anti-Ku70 antibody [EPR4027] - BSA and Azide free (ab239912)

    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Ku70 with purified ab92450 at 1/20 dilution (5 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92450).

  • Immunocytochemistry/ Immunofluorescence - Anti-Ku70 antibody [EPR4027] - BSA and Azide free (ab239912)
    Immunocytochemistry/ Immunofluorescence - Anti-Ku70 antibody [EPR4027] - BSA and Azide free (ab239912)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Ku70 with purified ab92450 at 1/250 dilution (0.46 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92450).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ku70 antibody [EPR4027] - BSA and Azide free (ab239912)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ku70 antibody [EPR4027] - BSA and Azide free (ab239912)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue sections labeling Ku70 with purified ab92450 at 1/250 dilution (0.46 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92450).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ku70 antibody [EPR4027] - BSA and Azide free (ab239912)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ku70 antibody [EPR4027] - BSA and Azide free (ab239912)

    Paraffin embedded Human testis tissue (A) or Human tonsil tissue (B) were labelled with ab92450 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92450).

  • Immunocytochemistry/ Immunofluorescence - Anti-Ku70 antibody [EPR4027] - BSA and Azide free (ab239912)
    Immunocytochemistry/ Immunofluorescence - Anti-Ku70 antibody [EPR4027] - BSA and Azide free (ab239912)
    Immunofluorescence staining of HeLa cells using ab92450 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92450).

  • Flow Cytometry - Anti-Ku70 antibody [EPR4027] - BSA and Azide free (ab239912)
    Flow Cytometry - Anti-Ku70 antibody [EPR4027] - BSA and Azide free (ab239912)

    Overlay histogram showing HeLa cells stained with ab92450 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92450, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92450).

  • Anti-Ku70 antibody [EPR4027] - BSA and Azide free (ab239912)
    Anti-Ku70 antibody [EPR4027] - BSA and Azide free (ab239912)

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