Anti-Ku70 antibody [2F7F5] (ab202022)
Key features and details
- Mouse monoclonal [2F7F5] to Ku70
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Rat, Human, Recombinant fragment
- Isotype: IgG1
Overview
-
Product name
Anti-Ku70 antibody [2F7F5]
See all Ku70 primary antibodies -
Description
Mouse monoclonal [2F7F5] to Ku70 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB HumanRecombinant fragment -
Immunogen
Recombinant fragment corresponding to Human Ku70 aa 1-250. Expressed in E. Coli.
Database link: P12956 -
Positive control
- Human Ku70 (aa: 6-214) recombinant protein; Ku70 (aa: 6-214)-hIgGFc transfected HEK293 cell lysate; HeLa, PC-2, A549, A431, HepG2 and K562 cell lysates; A431 cells; Human endometrial cancer and cervical cancer tissues
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
-
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
2F7F5 -
Isotype
IgG1 -
Research areas
Images
-
Anti-Ku70 antibody [2F7F5] (ab202022) at 1/500 dilution + Ku70 (aa: 6-214) recombinant protein. at 1 µg
Predicted band size: 70 kDaExpected MW is 49.7 kDa
-
All lanes : Anti-Ku70 antibody [2F7F5] (ab202022) at 1/500 dilution
Lane 1 : Non transfected HEK293 cell lysate.
Lane 2 : Ku70 (aa: 6-214)-hIgGFc transfected HEK293 cell lysate.
Lysates/proteins at 1 µg per lane.
Predicted band size: 70 kDa
-
All lanes : Anti-Ku70 antibody [2F7F5] (ab202022) at 1/500 dilution
Lane 1 : HeLa cell lysate
Lane 2 : PC-2 cell lysate
Lane 3 : A549 cell lysate
Lane 4 : A431 cell lysate
Lane 5 : HepG2 cell lysate
Lane 6 : K562 cell lysate
Lysates/proteins at 1 µg per lane.
Predicted band size: 70 kDa
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ku70 antibody [2F7F5] (ab202022)
Immunohistochemical analysis of paraffin embedded Human endometrial cancer tissues labeling Ku70 using ab202022 at 1/200 dilution, followed by DAB staining.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ku70 antibody [2F7F5] (ab202022)
Immunohistochemical analysis of paraffin embedded Human cervical cancer tissues labeling Ku70 using ab202022 at 1/200 dilution, followed by DAB staining.
-
Flow cytometric analysis of A431 cells labeling Ku70 using ab202022 (green) at 1/200 dilution, and negative control (red).