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Anti-Ku70 antibody [2F7F5] (ab202022)

Anti-Ku70 antibody [2F7F5] (ab202022)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [2F7F5] to Ku70
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Rat, Human, Recombinant fragment
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Ku70 antibody [2F7F5]
    See all Ku70 primary antibodies
  • Description

    Mouse monoclonal [2F7F5] to Ku70
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    Recombinant fragment
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human Ku70 aa 1-250. Expressed in E. Coli.
    Database link: P12956

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human Ku70 (aa: 6-214) recombinant protein; Ku70 (aa: 6-214)-hIgGFc transfected HEK293 cell lysate; HeLa, PC-2, A549, A431, HepG2 and K562 cell lysates; A431 cells; Human endometrial cancer and cervical cancer tissues
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    2F7F5
  • Isotype

    IgG1
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Non Homol. End Joining
    • Cancer
    • Cell cycle
    • Cell division

Images

  • Western blot - Anti-Ku70 antibody [2F7F5] (ab202022)
    Western blot - Anti-Ku70 antibody [2F7F5] (ab202022)
    Anti-Ku70 antibody [2F7F5] (ab202022) at 1/500 dilution + Ku70 (aa: 6-214) recombinant protein. at 1 µg

    Predicted band size: 70 kDa



    Expected MW is 49.7 kDa

  • Western blot - Anti-Ku70 antibody [2F7F5] (ab202022)
    Western blot - Anti-Ku70 antibody [2F7F5] (ab202022)
    All lanes : Anti-Ku70 antibody [2F7F5] (ab202022) at 1/500 dilution

    Lane 1 : Non transfected HEK293 cell lysate.
    Lane 2 : Ku70 (aa: 6-214)-hIgGFc transfected HEK293 cell lysate.

    Lysates/proteins at 1 µg per lane.

    Predicted band size: 70 kDa

  • Western blot - Anti-Ku70 antibody [2F7F5] (ab202022)
    Western blot - Anti-Ku70 antibody [2F7F5] (ab202022)
    All lanes : Anti-Ku70 antibody [2F7F5] (ab202022) at 1/500 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : PC-2 cell lysate
    Lane 3 : A549 cell lysate
    Lane 4 : A431 cell lysate
    Lane 5 : HepG2 cell lysate
    Lane 6 : K562 cell lysate

    Lysates/proteins at 1 µg per lane.

    Predicted band size: 70 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ku70 antibody [2F7F5] (ab202022)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ku70 antibody [2F7F5] (ab202022)

    Immunohistochemical analysis of paraffin embedded Human endometrial cancer tissues labeling Ku70 using ab202022 at 1/200 dilution, followed by DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ku70 antibody [2F7F5] (ab202022)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ku70 antibody [2F7F5] (ab202022)

    Immunohistochemical analysis of paraffin embedded Human cervical cancer tissues labeling Ku70 using ab202022 at 1/200 dilution, followed by DAB staining.

  • Flow Cytometry - Anti-Ku70 antibody [2F7F5] (ab202022)
    Flow Cytometry - Anti-Ku70 antibody [2F7F5] (ab202022)

    Flow cytometric analysis of A431 cells labeling Ku70 using ab202022 (green) at 1/200 dilution, and negative control (red).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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