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Anti-IKAP/p150 antibody [EPR13113] - BSA and Azide free (ab210396)

Anti-IKAP/p150 antibody [EPR13113] - BSA and Azide free (ab210396)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13113] to IKAP/p150 - BSA and Azide free
  • Suitable for: ICC, WB, Flow Cyt
  • Reacts with: Human

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Overview

  • Product name

    Anti-IKAP/p150 antibody [EPR13113] - BSA and Azide free
    See all IKAP/p150 primary antibodies
  • Description

    Rabbit monoclonal [EPR13113] to IKAP/p150 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, WB, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human IKAP/p150. The exact sequence is proprietary.
    Database link: O95163

  • Positive control

    • HepG2, HeLa, Jurkat and 293T cell lysates; HeLa cells; Jurkat cells.
  • General notes

    ab210396 is the carrier-free version of ab179437 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab210396 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as IKAP

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13113
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Adapters
    • Cytoplasmic
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • NFkB pathway

Images

  • Western blot - Anti-IKAP/p150 antibody [EPR13113] - BSA and Azide free (ab210396)
    Western blot - Anti-IKAP/p150 antibody [EPR13113] - BSA and Azide free (ab210396)
    All lanes : Anti-IKAP/p150 antibody [EPR13113] (ab179437) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : 293T (Human embryonic kidney epithelial cell) cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 150 kDa



    This data was developed using ab179437, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-IKAP/p150 antibody [EPR13113] - BSA and Azide free (ab210396)
    Immunocytochemistry - Anti-IKAP/p150 antibody [EPR13113] - BSA and Azide free (ab210396)
    This data was developed using ab179437, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells labeling IKAP/p150 with ab179437 at 1/50 dilution (green). DAPI nuclear staining (blue).
  • Flow Cytometry - Anti-IKAP/p150 antibody [EPR13113] - BSA and Azide free (ab210396)
    Flow Cytometry - Anti-IKAP/p150 antibody [EPR13113] - BSA and Azide free (ab210396)
    This data was developed using ab179437, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized Jurkat cells labeling IKAP/p150 with ab179437 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
  • Anti-IKAP/p150 antibody [EPR13113] - BSA and Azide free (ab210396)
    Anti-IKAP/p150 antibody [EPR13113] - BSA and Azide free (ab210396)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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