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Anti-Ku70 antibody [7A9E7] (ab201963)

Anti-Ku70 antibody [7A9E7] (ab201963)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [7A9E7] to Ku70
  • Suitable for: Flow Cyt, WB
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Ku70 antibody [7A9E7]
    See all Ku70 primary antibodies
  • Description

    Mouse monoclonal [7A9E7] to Ku70
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment corresponding to Human Ku70 aa 1-250. Expressed in E. Coli.
    Database link: P12956

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human recombinant Ku70 (aa6-214) protein; Human recombinant Ku70 (aa6-214)-hIgGFc transfected HEK293 cell lysate; PC2 , A549, A431, HepG and K562 cell lysates; A431 cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    7A9E7
  • Isotype

    IgG1
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Non Homol. End Joining
    • Cancer
    • Cell cycle
    • Cell division

Images

  • Flow Cytometry - Anti-Ku70 antibody [7A9E7] (ab201963)
    Flow Cytometry - Anti-Ku70 antibody [7A9E7] (ab201963)

    Flow cytometric analysis of A431 cells labeling Ku70 with ab201963 at 1/200 dilution (green). Negative control (red).

  • Western blot - Anti-Ku70 antibody [7A9E7] (ab201963)
    Western blot - Anti-Ku70 antibody [7A9E7] (ab201963)
    All lanes : Anti-Ku70 antibody [7A9E7] (ab201963) at 1/500 dilution

    Lane 1 : PC2 cell lysate
    Lane 2 : A549 cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : HepG2 cell lysate
    Lane 5 : K562 cell lysate

    Predicted band size: 70 kDa

  • Western blot - Anti-Ku70 antibody [7A9E7] (ab201963)
    Western blot - Anti-Ku70 antibody [7A9E7] (ab201963)
    All lanes : Anti-Ku70 antibody [7A9E7] (ab201963) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : Human recombinant Ku70 (aa6-214)-hIgGFc transfected HEK293 cell lysate

    Predicted band size: 70 kDa

  • Western blot - Anti-Ku70 antibody [7A9E7] (ab201963)
    Western blot - Anti-Ku70 antibody [7A9E7] (ab201963)
    Anti-Ku70 antibody [7A9E7] (ab201963) at 1/500 dilution + Human recombinant Ku70 (aa6-214) protein

    Predicted band size: 70 kDa



    (Expected MW is 49.7 kDa)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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