All lanes : Anti-MCL1 antibody [Y37] (ab32087) at 1/1000 dilution

Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : MCL1 knockout HEK293T cell lysate
Lane 3 : Ramos cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 37 kDa
Observed band size: 37 kDa

Lanes 1-3: Merged signal (red and green). Green - ab32087 observed at 37 kDa. Red - loading control ab7291 observed at 50 kDa. 

 ab32087  Anti-MCL1 antibody [Y37]  was shown to specifically react with MCL1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266838 (knockout cell lysate ab256986) was used.  Wild-type and MCL1 knockout samples were subjected to SDS-PAGE.  ab32087 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

Allele-1: 1 bp deletion in exon 1

 

Allele-2: 7 bp insertion in exon 1.