Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)
![Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)](https://www.abcam.com/ps/products/273/ab273434/Images/ab273434-392345-AntiSARS-Nucleoprotein-antibody-6H3-westernblot-HEK293T--cells.JPG "Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)")
Key features and details
- Mouse monoclonal [6H3] to SARS Nucleocapsid Protein
- Suitable for: ICC, WB, ELISA, IP, Sandwich ELISA
- Isotype: IgG1
Overview
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Product name
Anti-SARS Nucleocapsid Protein antibody [6H3]
See all SARS Nucleocapsid Protein primary antibodies -
Description
Mouse monoclonal [6H3] to SARS Nucleocapsid Protein -
Host species
Mouse -
Specificity
No cross-reactivity with MERS-CoV nucleocapsid protein is expected.
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Tested applications
Suitable for: ICC, WB, ELISA, IP, Sandwich ELISAmore details -
Species reactivity
Reacts with: SARS-CoV, SARS-CoV-2
Does not react with: MERS-CoV -
Immunogen
Recombinant fragment corresponding to Human coronavirus SARS Nucleocapsid Protein aa 121-422.
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Positive control
- IP: SARS CoV-2 Nucleocapsid Protein-transfected HEK-293T whole cell lysate. WB: SARS-CoV infected HEK-293T cell lysate; HEK-293T cells transfected with SARS CoV-2 Nucleocapsid Protein with DDDDK tag; Vero E6; SARS CoV and SARS CoV-2 infected Caco-2 cells whole cell extract. ICC: VeroE6 cells overexpressing SARS CoV Nucleocapsid Protein. SARS CoV-2 Nucleocapsid Protein transfected 293T cells.
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General notes
Applications overview
Tick: Tested and Guaranteed to work X: Will not work —: No data
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
6H3 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)All lanes : Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434) at 1/5000 dilution
Lane 1 : Non-transfected HEK-293T cells at 30 µg
Lane 2 : SARS-CoV NP transfected HEK-293T cells at 30 µg
Lane 3 : SARS-CoV2 (COVID-19) NP transfected HEK-293T cells
Lane 4 : MERS-CoV NP transfected HEK-293T cells at 30 µg10% SDS-PAGE
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![Immunocytochemistry/ Immunofluorescence - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)](https://www.abcam.com/ps/products/273/ab273434/Images/ab273434-392346-AntiSARS-Nucleoprotein-antibody-6H3-immunofluorescence-VeroE6-cells.JPG)
Immunocytochemistry/ Immunofluorescence - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)VeroE6 cells stained for SARS Nucleocapsid Protein (green) using ab273434 in ICC/IF.
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![Sandwich ELISA - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)](https://www.abcam.com/ps/products/273/ab273434/Images/ab273434-392339-AntiSARS-Nucleoprotein-antibody-6H3-Sandwich-ELISA.JPG)
Sandwich ELISA - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)Sandwich ELISA detection of recombinant full-length SARS-CoV-2 nucleocapsid protein using a different anti-SARS-CoV-2 capture antibody at concentration of 5 μg/mL and ab273434 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) was used at 1/10000 to detect the primary antibody.
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![Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)](https://www.abcam.com/ps/products/273/ab273434/Images/ab273434-392344-ab273434392344AntiSARSNucleoproteinantibody6H3westernblotSARSCoV2COVID19nucleocapsidprotein.jpg)
Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434) at 1/5000 dilution + SARS-CoV-2 (COVID-19) nucleocapsid protein at 0.5 µg
Secondary
HRP-conjugated anti-Mouse IgG12% SDS-PAGE
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![Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)](https://www.abcam.com/ps/products/273/ab273434/Images/ab273434-392343-AntiSARS-Nucleoprotein-antibody-6H3-westernblot-HEK293Tcells.JPG)
Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)All lanes : Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434) at 1/5000 dilution
Lane 1 : Non-transfected HEK-293T whole cell extract
Lane 2 : SARS-CoV nucleocapsid-transfected (a partial fragment) HEK-293T whole cell extracts
Lysates/proteins at 30 µg per lane.12% SDS-PAGE
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![Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)](https://www.abcam.com/ps/products/273/ab273434/Images/ab273434-392341-AntiSARS-Nucleoprotein-antibody-6H3-westernblot-HEK293T-cells.JPG)
Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)All lanes : Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434) at 1/5000 dilution
Lane 1 : Non-transfected HEK-293T whole cell extract
Lane 2 : Transfected HEK-293T whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-Mouse IgG10% SDS-PAGE
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![Immunoprecipitation - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)](https://www.abcam.com/ps/products/273/ab273434/Images/ab273434-392340-ab273434392340AntiSARSNucleoproteinantibody6H3ImmunoprecipitationHEK293Tcells.jpg)
Immunoprecipitation - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)SARS Nucleocapsid Protein was immunoprecipitated from 2μg of HEK-293T whole cell lysate with either ab273434 (Lane 3) or a control IgG (Lane 2).
Western blot was performed from the immunoprecipitate using ab273434. An
HRP-conjugated anti mouse IgG antibody, was used as secondary antibody.
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![Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)](https://www.abcam.com/ps/products/273/ab273434/Images/ab273434-392342-AntiSARS-Nucleoprotein-antibody-6H3-westernblot-Vero-E6-cells.JPG)
Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)All lanes : Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)
Lane 1 : SARS-CoV infected Vero E6 cells post infection after 0 hours
Lane 2 : SARS-CoV infected Vero E6 cells post infection after 8 hours
Lane 3 : SARS-CoV infected Vero E6 cells post infection after 16 hours
Lane 4 : SARS-CoV infected Vero E6 cells post infection after 24 hoursVero E6 cells were analysed at the indicated time-points post-infection with SARS-CoV.
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![Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)](https://www.abcam.com/ps/products/273/ab273434/Images/ab273434-392347-AntiSARS-Nucleoprotein-antibody-6H3-westernblot-Caco2-cells.JPG)
Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)Lanes 1-2 : Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434) at 1/1000 dilution
Lane 3 : Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)
Lane 1 : Non-infected Caco-2 whole cell extract
Lane 2 : SARS-CoV infected Caco-2 whole cell extract
Lane 3 : SARS-CoV-2 infected Caco-2 whole cell extractCell lysates were analysed by WB 48 hours post-infection with SARS-CoV or SARS Cov-2 at a MOI of 0.01.
