Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BAZ2B antibody [EPR20909-88] (ab234420)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20909-88] to BAZ2B
  • Suitable for: Flow Cyt, WB, ICC
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-BAZ2B antibody [EPR20909-88]
    See all BAZ2B primary antibodies

  • Description

    Rabbit monoclonal [EPR20909-88] to BAZ2B

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T transfected with Baz2b expression vector containig a HA-tag, whole cell lysate; Fetal mouse brain tissue lysate. ICC: HEK-293T cells. Flow cyt: HEK-293T cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20909-88

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-BAZ2B antibody [EPR20909-88] (ab234420)
    Western blot - Anti-BAZ2B antibody [EPR20909-88] (ab234420)
    All lanes : Anti-BAZ2B antibody [EPR20909-88] (ab234420) at 1/1000 dilution

    Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a HA-tag, whole cell lysate
    Lane 2 : HEK-293T transfected with Baz2b expression vector containing a HA-tag, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 234 kDa
    Observed band size: 234 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes. 

  • Immunocytochemistry - Anti-BAZ2B antibody [EPR20909-88] (ab234420)
    Immunocytochemistry - Anti-BAZ2B antibody [EPR20909-88] (ab234420)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T cells labelling BAZ2B with ab234420 at 1/2000 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 (2µg/ml) dilution (Green). Confocal image showing nuclear staining in HEK-293T cells transfected with 3HA-tagged Baz2b expression vector. Anti-HA.11 Epitope Tag mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2µg/ml) dilution.

  • Flow Cytometry - Anti-BAZ2B antibody [EPR20909-88] (ab234420)
    Flow Cytometry - Anti-BAZ2B antibody [EPR20909-88] (ab234420)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HEK-293T (Human embryonic kidney epithelial cell) (transfected with HA-tagged Baz2b expression vector) cells labelling BAZ2B with ab234420 at 1/600 dilution (0.1µg) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-BAZ2B antibody [EPR20909-88] (ab234420)
    Western blot - Anti-BAZ2B antibody [EPR20909-88] (ab234420)
    Anti-BAZ2B antibody [EPR20909-88] (ab234420) at 1/500 dilution + Mouse fetal brain at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 234 kDa
    Observed band size: 234 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 62 seconds.

  • Anti-BAZ2B antibody [EPR20909-88] (ab234420)
    Anti-BAZ2B antibody [EPR20909-88] (ab234420)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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