Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸ 10 µl 90 460 ₸

Order now and get it on Wednesday March 10, 2021

Anti-BAZ2B antibody [EPR20909-88] (ab234420)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20909-88] to BAZ2B
Suitable for: Flow Cyt, WB, ICC
Reacts with: Mouse, Human

Product name

Anti-BAZ2B antibody [EPR20909-88]
See all BAZ2B primary antibodies
Description

Rabbit monoclonal [EPR20909-88] to BAZ2B
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HEK-293T transfected with Baz2b expression vector containig a HA-tag, whole cell lysate; Fetal mouse brain tissue lysate. ICC: HEK-293T cells. Flow cyt: HEK-293T cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20909-88
Isotype

IgG
Research areas

Western blot - Anti-BAZ2B antibody [EPR20909-88] (ab234420)

All lanes : Anti-BAZ2B antibody [EPR20909-88] (ab234420) at 1/1000 dilution

Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a HA-tag, whole cell lysate
Lane 2 : HEK-293T transfected with Baz2b expression vector containing a HA-tag, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Predicted band size: 234 kDa
Observed band size: 234 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes.
Immunocytochemistry - Anti-BAZ2B antibody [EPR20909-88] (ab234420)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T cells labelling BAZ2B with ab234420 at 1/2000 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 (2µg/ml) dilution (Green). Confocal image showing nuclear staining in HEK-293T cells transfected with 3HA-tagged Baz2b expression vector. Anti-HA.11 Epitope Tag mouse monoclonal antibody (Alexa Fluor^® 647) was used to counterstain tubulin at 1/100 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 (2µg/ml) dilution.
Flow Cytometry - Anti-BAZ2B antibody [EPR20909-88] (ab234420)

Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HEK-293T (Human embryonic kidney epithelial cell) (transfected with HA-tagged Baz2b expression vector) cells labelling BAZ2B with ab234420 at 1/600 dilution (0.1µg) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Western blot - Anti-BAZ2B antibody [EPR20909-88] (ab234420)

Anti-BAZ2B antibody [EPR20909-88] (ab234420) at 1/500 dilution + Mouse fetal brain at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

Predicted band size: 234 kDa
Observed band size: 234 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Exposure time: 62 seconds.
Anti-BAZ2B antibody [EPR20909-88] (ab234420)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com