Anti-DEK antibody (ab105175)
Key features and details
- Rabbit polyclonal to DEK
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DEK antibody
See all DEK primary antibodies -
Description
Rabbit polyclonal to DEK -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human DEK aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab116620) -
Positive control
- This antibody gave a positive signal in Human brain tissue lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab105175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications RatHorseCowDogPigMacaque monkeyApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).Target
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Function
Involved in chromatin organization. -
Tissue specificity
Ubiquitous. Expressed at relatively high levels. -
Involvement in disease
A chromosomal aberration involving DEK is found in a subset of acute myeloid leukemia (AML); also known as acute non-lymphocytic leukemia (PubMed:1549122). Translocation t(6;9)(p23;q34) with NUP214/CAN (PubMed:1549122). It results in the formation of a DEK-NUP214 fusion gene (PubMed:1549122). -
Sequence similarities
Contains 1 SAP domain. -
Post-translational
modificationsPhosphorylated by CK2. Phosphorylation fluctuates during the cell cycle with a moderate peak during G(1) phase, and weakens the binding of DEK to DNA. -
Cellular localization
Nucleus. Enriched in regions where chromatin is decondensed or sparse in the interphase nuclei. - Information by UniProt
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Database links
- Entrez Gene: 7913 Human
- Entrez Gene: 306817 Rat
- Omim: 125264 Human
- SwissProt: P35659 Human
- SwissProt: Q6AXS3 Rat
- Unigene: 484813 Human
- Unigene: 25099 Rat
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Alternative names
- D6S231E antibody
- Dek antibody
- DEK gene antibody
see all
Images
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Western blot - Anti-DEK antibody (ab105175)Anti-DEK antibody (ab105175) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 8 minutes
Datasheets and documents
References (0)
ab105175 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-DEK antibody (ab105175)Anti-DEK antibody (ab105175) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 8 minutes
