Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human MICA ELISA Kit (ab100592)

Key features and details

  • Sensitivity: 20 pg/ml
  • Range: 13.72 pg/ml - 10000 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human MICA ELISA Kit
    See all MICA kits

  • Detection method

    Colorimetric

  • Sample type

    Cell culture supernatant, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

  • Range

    13.72 pg/ml - 10000 pg/ml

  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 116.9 109% - 124%
    Serum 112.3 95% - 129%
    Plasma 93.83 75% - 107%

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Abcam’s MICA Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of Human MICA in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human MICA coated on a 96-well plate. Standards and samples are pipetted into the wells and MICA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MICA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MICA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    300X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent 1 x 15ml
    Biotinylated anti-Human MICA (lyophilized) 2 vials
    MICA Microplate (12 x 8 wells) 1 unit
    Recombinant Human MICA Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml

  • Research areas

  • Relevance

    The MHC class I chain-related (MIC) proteins are related to the Major histocompatibility complex (MHC) class I proteins which are ubiquitously expressed and mediate the recognition of intracellular antigens by cytotoxic T cells. The MHC class I chain-related (MIC) proteins are recognized by NKG2D, a receptor on NK and T cells, and promote anti-tumor activity. MICA, a member of the MIC family, is widely expressed on many tumors, and it is the MICA/NKG2D interaction that is thought to stimulate the anti-tumor reactivity by T lymphocytes. MICA is present in virtually every tissue except the nervous system, suggesting that MIC protein expression may only be one component of the anti-tumor activity of the immune system.

  • Cellular localization

    Plasma membrane

  • Alternative names

    • FLJ60820
    • HLA class I antigen
    • MGC111087
    • MHC class I chain related gene A protein
    • MHC class I chain related protein A
    • MHC class I polypeptide related sequence A
    • MHC class I related protein
    • MIC A
    • PERB11.1
    • Stress inducible class I homolog
    see all

  • Database links

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 1 x 96 tests
      20X Wash Buffer Concentrate 1 x 25ml
      300X HRP-Streptavidin Concentrate 1 x 200µl
      5X Assay Diluent 1 x 15ml
      Biotinylated anti-Human MICA (lyophilized) 2 vials
      MICA Microplate (12 x 8 wells) 1 unit
      Recombinant Human MICA Standard (lyophilized) 2 vials
      Stop Solution 1 x 8ml
      TMB One-Step Substrate Reagent 1 x 12ml

    • Research areas

    • Relevance

      The MHC class I chain-related (MIC) proteins are related to the Major histocompatibility complex (MHC) class I proteins which are ubiquitously expressed and mediate the recognition of intracellular antigens by cytotoxic T cells. The MHC class I chain-related (MIC) proteins are recognized by NKG2D, a receptor on NK and T cells, and promote anti-tumor activity. MICA, a member of the MIC family, is widely expressed on many tumors, and it is the MICA/NKG2D interaction that is thought to stimulate the anti-tumor reactivity by T lymphocytes. MICA is present in virtually every tissue except the nervous system, suggesting that MIC protein expression may only be one component of the anti-tumor activity of the immune system.

    • Cellular localization

      Plasma membrane

    • Alternative names

      • FLJ60820
      • HLA class I antigen
      • MGC111087
      • MHC class I chain related gene A protein
      • MHC class I chain related protein A
      • MHC class I polypeptide related sequence A
      • MHC class I related protein
      • MIC A
      • PERB11.1
      • Stress inducible class I homolog
      see all

    • Database links

      Images

      • ELISA: MICA Human ELISA Kit (ab100592)
        ELISA: MICA Human ELISA Kit (ab100592)

        Standard curve with background signal subtracted (duplicates; +/- SD).

      • ELISA: MICA Human ELISA Kit (ab100592)
        ELISA: MICA Human ELISA Kit (ab100592)

        MICA measured in undiluted cell culture supernatants, U937 and THP-1 signals were below level of detection (13.7 pg x mL-1) (duplicates +/- SD).

      • Typical Standard Curve
        Typical Standard Curve

        Representative Standard Curve using ab100592.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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