Anti-HLA Class I antibody [W6/32] (ab22432)
![Anti-HLA Class I antibody [W6/32] (ab22432)](https://www.abcam.com/ps/products/22/ab22432/Images/ab22432-346693-anti-hla-class-1-w632-antibody-flow-cytometry-human-blood-lymphoctyes.jpg "Anti-HLA Class I antibody [W6/32] (ab22432)")
Key features and details
- Mouse monoclonal [W6/32] to HLA Class I
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-HLA Class I antibody [W6/32]
See all HLA Class I primary antibodies -
Description
Mouse monoclonal [W6/32] to HLA Class I -
Host species
Mouse -
Specificity
Recognises an antigenic determinant shared among products of the HLA A, B and C loci. The antibody recognises virtually all nucleated human cells. It recognises an antigenic determinant shared among products of the HLA A, B and C loci. Clone W6/32 recognises a conformational epitope, reacting with HLA class I alpha3 and alpha2 domains. -
Tested applications
Suitable for: Flow Cytmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Chimpanzee, Baboon, Cynomolgus monkey, Macaque monkey, Rhesus monkey, Gorilla
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Immunogen
Tissue, cells or virus corresponding to Human HLA Class I. Purified human tonsil lymphocyte membranes.
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Positive control
- Flow Cyt: Human peripheral blood lymphocytes.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
W6/32 -
Myeloma
NS1/1-Ag4-1 -
Isotype
IgG2a -
Research areas
Images
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Flow Cytometry - Anti-HLA Class I antibody [W6/32] (ab22432)
Flow cytometric analysis of red cell lysed human blood gated on lymphocytes in the presence of Human seroblock using ab22432 at 1/50 dilution (Figure B) compared to anPacific Blue conjugated Mouse IgG2a isotype control (Figure A).
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![Flow Cytometry - Anti-HLA Class I antibody [W6/32] (ab22432)](https://www.abcam.com/ps/products/22/ab22432/Images/ab22432-346692-anti-hla-class-1-w632-antibody-flow-cytometry-human-blood.jpg)
Flow Cytometry - Anti-HLA Class I antibody [W6/32] (ab22432)Flow cytometric analysis of red cell lysed human blood gated on lymphocytes using ab22432 at 1/50 dilution (Figure B) compared to an isotype control (Figure A).
