Anti-TPN antibody [7F6] - BSA and Azide free (ab255872)
![Anti-TPN antibody [7F6] - BSA and Azide free (ab255872)](https://www.abcam.com/ps/products/255/ab255872/Images/ab255872-390679-anti-tpn-antibody-7f6-western-blot-hepg2.jpg "Anti-TPN antibody [7F6] - BSA and Azide free (ab255872)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [7F6] to TPN - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-TPN antibody [7F6] - BSA and Azide free
See all TPN primary antibodies -
Description
Rat monoclonal [7F6] to TPN - BSA and Azide free -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HepG2, HUVEC, Jurkat, Daudi, Raji and HeLa whole lysates.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
ab255872 is the carrier-free version of ab252869. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
7F6 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-TPN antibody [7F6] - BSA and Azide free (ab255872)All lanes : Anti-TPN antibody [7F6] (ab252869) at 1/1000 dilution
Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lane 2 : HUVEC (human umbilical vein endothelial cell), whole cell lysate
Lane 3 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 4 : Daudi (human Burkitts lymphoma lymphoblast), whole cell lysate
Lane 5 : Raji (human Burkitts lymphoma B lymphocyte), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution
Predicted band size: 48 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The double bands observed in lane 5 is consistent with what has been described in the literatures (PMID: 29107940, 17620360).
Exposure time: lane 1-3: 6 seconds; lane 4-5: 3 seconds.
This image was produced using the same antibody clone but in a different formulation ab252869, PBS, sodium azide, glycerol and BSA.
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![Western blot - Anti-TPN antibody [7F6] - BSA and Azide free (ab255872)](https://www.abcam.com/ps/products/255/ab255872/Images/ab255872-390680-anti-tpn-antibody-7f6-western-blot-hela.jpg)
Western blot - Anti-TPN antibody [7F6] - BSA and Azide free (ab255872)All lanes : Anti-TPN antibody [7F6] (ab252869) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : HeLa treated with 100 U/ml IFN gamma for 24 hours, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution
Predicted band size: 48 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
This image was produced using the same antibody clone but in a different formulation ab252869, PBS, sodium azide, glycerol and BSA.
-
![Anti-TPN antibody [7F6] - BSA and Azide free (ab255872)](https://www.abcam.com/ps/products/255/ab255872/Images/ab255872-3-benefits-of-recombinant-antibodies.png)
Anti-TPN antibody [7F6] - BSA and Azide free (ab255872)
