Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TPN antibody [7F6] - BSA and Azide free (ab255872)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [7F6] to TPN - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-TPN antibody [7F6] - BSA and Azide free
    See all TPN primary antibodies

  • Description

    Rat monoclonal [7F6] to TPN - BSA and Azide free

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2, HUVEC, Jurkat, Daudi, Raji and HeLa whole lysates.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab255872 is the carrier-free version of ab252869. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-TPN antibody [7F6] - BSA and Azide free (ab255872)
    Western blot - Anti-TPN antibody [7F6] - BSA and Azide free (ab255872)
    All lanes : Anti-TPN antibody [7F6] (ab252869) at 1/1000 dilution

    Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
    Lane 2 : HUVEC (human umbilical vein endothelial cell), whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
    Lane 4 : Daudi (human Burkitts lymphoma lymphoblast), whole cell lysate
    Lane 5 : Raji (human Burkitts lymphoma B lymphocyte), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

    Predicted band size: 48 kDa
    Observed band size: 47 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The double bands observed in lane 5 is consistent with what has been described in the literatures (PMID: 29107940, 17620360). 

    Exposure time: lane 1-3: 6 seconds; lane 4-5: 3 seconds.

    This image was produced using the same antibody clone but in a different formulation ab252869, PBS, sodium azide, glycerol and BSA.

  • Western blot - Anti-TPN antibody [7F6] - BSA and Azide free (ab255872)
    Western blot - Anti-TPN antibody [7F6] - BSA and Azide free (ab255872)
    All lanes : Anti-TPN antibody [7F6] (ab252869) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : HeLa treated with 100 U/ml IFN gamma for 24 hours, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

    Predicted band size: 48 kDa
    Observed band size: 47 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    This image was produced using the same antibody clone but in a different formulation ab252869, PBS, sodium azide, glycerol and BSA.

  • Anti-TPN antibody [7F6] - BSA and Azide free (ab255872)
    Anti-TPN antibody [7F6] - BSA and Azide free (ab255872)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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