Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CLPP antibody [EPR7133] - BSA and Azide free (ab236064)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7133] to CLPP - BSA and Azide free
  • Suitable for: IHC-P, IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CLPP antibody [EPR7133] - BSA and Azide free
    See all CLPP primary antibodies

  • Description

    Rabbit monoclonal [EPR7133] to CLPP - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Fetal muscle lysate; Saos-2, A431, HEK-293T, K562 and A549 cell lysates.

  • General notes

    Ab236064 is the carrier-free version of ab124822. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab236064 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-CLPP antibody [EPR7133] - BSA and Azide free (ab236064)
    Western blot - Anti-CLPP antibody [EPR7133] - BSA and Azide free (ab236064)
    All lanes : Anti-CLPP antibody [EPR7133] (ab124822) at 1/1000 dilution

    Lane 1 : Fetal muscle lysate
    Lane 2 : Saos-2 (Human osteosarcoma cell line) cell lysate
    Lane 3 : A431 (Human epidermoid carcinoma cell line) cell lysate
    Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 6 : A549 (Human lung carcinoma cell line) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 30 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124822).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLPP antibody [EPR7133] - BSA and Azide free (ab236064)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLPP antibody [EPR7133] - BSA and Azide free (ab236064)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat stomach tissue sections labeling CLPP with Purified ab124822 at 1:50 dilution (2 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124822).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLPP antibody [EPR7133] - BSA and Azide free (ab236064)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLPP antibody [EPR7133] - BSA and Azide free (ab236064)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse kidney tissue sections labeling CLPP with Purified ab124822 at 1:50 dilution (2 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124822).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLPP antibody [EPR7133] - BSA and Azide free (ab236064)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLPP antibody [EPR7133] - BSA and Azide free (ab236064)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling CLPP with Purified ab124822 at 1:50 dilution (2 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124822).

  • Immunoprecipitation - Anti-CLPP antibody [EPR7133] - BSA and Azide free (ab236064)
    Immunoprecipitation - Anti-CLPP antibody [EPR7133] - BSA and Azide free (ab236064)

    ab124822 (purified) at 1:20 dilution (2µg) immunoprecipitating CLPP in K562 whole cell lysate.
    Lane 1 (input): K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10µg
    Lane 2 (+): ab124822 & K562 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124822 in K562 whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:5000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124822).

  • Anti-CLPP antibody [EPR7133] - BSA and Azide free (ab236064)
    Anti-CLPP antibody [EPR7133] - BSA and Azide free (ab236064)

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