Anti-HLA F antibody [EPR6803] - BSA and Azide free (ab248133)
![Anti-HLA F antibody [EPR6803] - BSA and Azide free (ab248133)](https://www.abcam.com/ps/products/248/ab248133/Images/ab248133-3-anti-hla-f-antibody-epr6803-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg "Anti-HLA F antibody [EPR6803] - BSA and Azide free (ab248133)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6803] to HLA F - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-HLA F antibody [EPR6803] - BSA and Azide free
See all HLA F primary antibodies -
Description
Rabbit monoclonal [EPR6803] to HLA F - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab248133 is the carrier-free version of ab126624 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab248133 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 2.52 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR6803 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA F antibody [EPR6803] - BSA and Azide free (ab248133)This data was developed using ab126624, the same antibody clone in a different buffer formulation.ab126624, at a 1/250 dilution, staining HLA F in paraffin-embedded Human tonsil tissue by immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-HLA F antibody [EPR6803] - BSA and Azide free (ab248133)](https://www.abcam.com/ps/products/248/ab248133/Images/ab248133-402220-anti-hla-f-antibody-epr6803-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-HLA F antibody [EPR6803] - BSA and Azide free (ab248133)All lanes : Anti-HLA F antibody [EPR6803] (ab126624) at 1/1000 dilution
Lane 1 : HT1376 cell lysate
Lane 2 : Human tonsil lysate
Lane 3 : JAR cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 39 kDaThis data was developed using ab126624, the same antibody clone in a different buffer formulation.
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![OI-RD Scanning - Anti-HLA F antibody [EPR6803] - BSA and Azide free (ab248133)](https://www.abcam.com/ps/products/248/ab248133/Images/ab248133-1-anti-hla-f-antibody-epr6803-bsa-and-azide-free-equilibrium-disassociation-constant-kd.jpg)
OI-RD Scanning - Anti-HLA F antibody [EPR6803] - BSA and Azide free (ab248133)This data was developed using ab126624, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
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![Anti-HLA F antibody [EPR6803] - BSA and Azide free (ab248133)](https://www.abcam.com/ps/products/248/ab248133/Images/ab248133-4-benefits-of-recombinant-antibodies.png)
Anti-HLA F antibody [EPR6803] - BSA and Azide free (ab248133)