Anti-Annexin A1/ANXA1 antibody [EPR2767(2)] - BSA and Azide free (ab248856)
![Anti-Annexin A1/ANXA1 antibody [EPR2767(2)] - BSA and Azide free (ab248856)](https://www.abcam.com/ps/products/248/ab248856/Images/ab248856-403395-anti-annexin-a1-anxa1-antibody-epr2767-2-bsa-and-azide-free-western-blot.jpg "Anti-Annexin A1/ANXA1 antibody [EPR2767(2)] - BSA and Azide free (ab248856)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2767(2)] to Annexin A1/ANXA1 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Annexin A1/ANXA1 antibody [EPR2767(2)] - BSA and Azide free
See all Annexin A1/ANXA1 primary antibodies -
Description
Rabbit monoclonal [EPR2767(2)] to Annexin A1/ANXA1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab248856 is the carrier-free version of ab138512. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab248856 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR2767(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Annexin A1/ANXA1 antibody [EPR2767(2)] - BSA and Azide free (ab248856)
This data was developed using ab138512, the same antibody clone in a different buffer formulation.
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Annexin I knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab138512 observed at 40 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab138512 was shown to specifically react with Annexin I when Annexin I knockout samples were used. Wild-type and Annexin I knockout samples were subjected to SDS-PAGE. Ab138512 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Annexin A1/ANXA1 antibody [EPR2767(2)] - BSA and Azide free (ab248856)](https://www.abcam.com/ps/products/248/ab248856/Images/ab248856-398442-anti-annexin-a1-anxa1-antibody-epr2767-2-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Annexin A1/ANXA1 antibody [EPR2767(2)] - BSA and Azide free (ab248856)All lanes : Anti-Annexin A1/ANXA1 antibody [EPR2767(2)] (ab138512) at 1/1000 dilution
Lane 1 : C6 cell lysate
Lane 2 : Raw264.7 cell lysate
Lane 3 : NIH3T3 cell lysate
Lane 4 : C2C12 cell lysate
Lane 5 : K562 cell lysate
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 39 kDaThis data was developed using ab138512, the same antibody clone in a different buffer formulation.
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![Anti-Annexin A1/ANXA1 antibody [EPR2767(2)] - BSA and Azide free (ab248856)](https://www.abcam.com/ps/products/248/ab248856/Images/ab248856-3-benefits-of-recombinant-antibodies.png)
Anti-Annexin A1/ANXA1 antibody [EPR2767(2)] - BSA and Azide free (ab248856)
