Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Annexin A1/ANXA1 antibody [EPR2767(2)] - BSA and Azide free (ab248856)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2767(2)] to Annexin A1/ANXA1 - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Annexin A1/ANXA1 antibody [EPR2767(2)] - BSA and Azide free
    See all Annexin A1/ANXA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR2767(2)] to Annexin A1/ANXA1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab248856 is the carrier-free version of ab138512. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248856 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Images

  • Western blot - Anti-Annexin A1/ANXA1 antibody [EPR2767(2)] - BSA and Azide free (ab248856)
    Western blot - Anti-Annexin A1/ANXA1 antibody [EPR2767(2)] - BSA and Azide free (ab248856)

    This data was developed using ab138512, the same antibody clone in a different buffer formulation.

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)

    Lane 2: Annexin I knockout HAP1 whole cell lysate (20 µg)

    Lane 3: HeLa whole cell lysate (20 µg)

    Lanes 1 - 3: Merged signal (red and green). Green - ab138512 observed at 40 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab138512 was shown to specifically react with Annexin I when Annexin I knockout samples were used. Wild-type and Annexin I knockout samples were subjected to SDS-PAGE. Ab138512 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Annexin A1/ANXA1 antibody [EPR2767(2)] - BSA and Azide free (ab248856)
    Western blot - Anti-Annexin A1/ANXA1 antibody [EPR2767(2)] - BSA and Azide free (ab248856)
    All lanes : Anti-Annexin A1/ANXA1 antibody [EPR2767(2)] (ab138512) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : Raw264.7 cell lysate
    Lane 3 : NIH3T3 cell lysate
    Lane 4 : C2C12 cell lysate
    Lane 5 : K562 cell lysate

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 39 kDa



    This data was developed using ab138512, the same antibody clone in a different buffer formulation.

     

  • Anti-Annexin A1/ANXA1 antibody [EPR2767(2)] - BSA and Azide free (ab248856)
    Anti-Annexin A1/ANXA1 antibody [EPR2767(2)] - BSA and Azide free (ab248856)

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