Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] - BSA and Azide free (ab251571)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19680] to ENO1 + ENO2 + ENO3 - BSA and Azide free
- Suitable for: IHC-P, Flow Cyt, WB, ICC
- Reacts with: Mouse, Human
Overview
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Product name
Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] - BSA and Azide free
See all ENO1 + ENO2 + ENO3 primary antibodies -
Description
Rabbit monoclonal [EPR19680] to ENO1 + ENO2 + ENO3 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, Flow Cyt, WB, ICCmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251571 is the carrier-free version of ab222514. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251571 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Clonality
Monoclonal -
Clone number
EPR19680 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (ab222514) at 1/1000 dilution
Lane 1 : His-tagged human ENO1 (aa2-434) recombinant protein, 10 ng
Lane 2 : His-tagged human ENO2 (aa1-434) recombinant protein, 10 ng
Lane 3 : His-tagged human ENO3 (aa1-434) recombinant protein, 10 ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 47 kDa why is the actual band size different from the predicted?This data was developed using ab222514, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times: Lanes 1-2: 3 seconds; Lane 3: 1 seconds.
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Lanes 1-3 : Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (ab222514) at 1/2000 dilution
Lane 4 : Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (ab222514) at 1/10000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 3 : Human skeletal muscle
Lane 4 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 47 kDa why is the actual band size different from the predicted?This data was developed using ab222514, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times: Lanes 1, 2, and 4: 1 seconds; Lane 3: 3 seconds.
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All lanes : Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (ab222514) at 1/2000 dilution
Lane 1 : Human fetal heart
Lane 2 : Human fetal kidney
Lane 3 : Human fetal spleen
Lane 4 : Human fetal brain
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 47 kDa why is the actual band size different from the predicted?This data was developed using ab222514, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times: Lanes 1-3: 3 seconds; Lane 4: 1 seconds.
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All lanes : Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (ab222514) at 1/2000 dilution
Lane 1 : Mouse brain
Lane 2 : Mouse spleen
Lane 3 : Mouse kidney
Lane 4 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lane 5 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 47 kDa why is the actual band size different from the predicted?This data was developed using ab222514, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times: Lanes 1, 2, and 4: 3 minutes; Lane 3: 1 minute; Lane 5: 3 seconds.
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This data was developed using ab222514, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin embedded human pancreas tissue labeling ENO1 + ENO2 + ENO3 with ab222514 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic and nuclear staining on medium-small ducts of human pancreas (PMID: 19425054). Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol. -
This data was developed using ab222514, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded human clear cell carcinoma tissue labeling ENO1 + ENO2 + ENO3 with ab222514 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous and nuclear staining on human clear cell kidney carcinoma is observed. Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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This data was developed using ab222514, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded mouse pancreas tissue labeling ENO1 + ENO2 + ENO3 with ab222514 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic and nuclear staining on the medium-small ducts of normal mouse pancreas is observed. Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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This data was developed using ab222514, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 100% methanol fixed HeLa (human cervix adenocarcinoma epithelial cell) cells labeling ENO1 + ENO2 + ENO3 with ab222514 at 1/500 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line. Counterstains: ab195889 Anti-alpha Tubulin antibody [DM1A] (red) - Microtubule Marker (Alexa Fluor® 594) (1/200). DAPI (blue).
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This data was developed using ab222514, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 100% methanol fixed MCF7 (human breast adenocarcinoma epithelial cell) cells labeling ENO1 + ENO2 + ENO3 with ab222514 at 1/500 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on MCF7 cell line. Counterstains: ab195889 Anti-alpha Tubulin antibody [DM1A] (red) - Microtubule Marker (Alexa Fluor® 594) (1/200). DAPI (blue).
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This data was developed using ab222514, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde fixed HeLa (human cervix adenocarcinoma epithelial cell) cell line labeling ENO1 + ENO2 + ENO3 with ab222514 at 1/800 (red) compared with an isotype control Rabbit monoclonal IgG (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Secondary antibody Goat anti rabbit IgG Alexa Fluor® 488 (ab150077), at 1/2000 dilution was used as the secondary antibody.
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