Anti-ADRM1/ARM-1 antibody [EPR11450(B)] - BSA and Azide free (ab249319)
![Anti-ADRM1/ARM-1 antibody [EPR11450(B)] - BSA and Azide free (ab249319)](https://www.abcam.com/ps/products/249/ab249319/Images/ab249319-410173-anti-adrm1arm-1-antibody-epr11450-western-blot-human-knockout-lysate.jpg "Anti-ADRM1/ARM-1 antibody [EPR11450(B)] - BSA and Azide free (ab249319)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11450(B)] to ADRM1/ARM-1 - BSA and Azide free
- Suitable for: WB, IHC-P, Flow Cyt
- Knockout validated
- Reacts with: Mouse, Human
Overview
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Product name
Anti-ADRM1/ARM-1 antibody [EPR11450(B)] - BSA and Azide free
See all ADRM1/ARM-1 primary antibodies -
Description
Rabbit monoclonal [EPR11450(B)] to ADRM1/ARM-1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEk293T, K-562, Raji, HeLa, NIH/3T3, RAW 264.7 cell lysates. IHC-P: Human thyroid gland carcinoma and testis tissues. Flow Cyt: HeLa cells.
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General notes
ab249319 is the carrier-free version of ab157218 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab249319 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information. This product was previously labelled as ADRM1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR11450(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ADRM1/ARM-1 antibody [EPR11450(B)] - BSA and Azide free (ab249319)All lanes : Anti-ADRM1/ARM-1 antibody [EPR11450(B)] (ab157218) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : ADRM1 knockout HEK293T cell lysate
Lane 3 : K-562 cell lysate
Lane 4 : Raji cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDaThis data was developed using ab157218, the same antibody clone in a different buffer formulation.
Lanes 1-4: Merged signal (red and green). Green - ab157218 observed at 42 kDa. Red - loading control ab8245 observed at 36 kDa.
ab157218 Anti-ADRM1/ARM-1 antibody [EPR11450(B)] was shown to specifically react with ADRM1/ARM-1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266765 (knockout cell lysate ab257816) was used. Wild-type and ADRM1/ARM-1 knockout samples were subjected to SDS-PAGE. ab157218 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRM1/ARM-1 antibody [EPR11450(B)] - BSA and Azide free (ab249319)](https://www.abcam.com/ps/products/249/ab249319/Images/ab249319-3-anti-adrm1-arm-1-antibody-epr11450-b-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRM1/ARM-1 antibody [EPR11450(B)] - BSA and Azide free (ab249319)This data was developed using ab157218, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human testis tissue labeling ADRM1/ARM-1 with ab157218 at 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Western blot - Anti-ADRM1/ARM-1 antibody [EPR11450(B)] - BSA and Azide free (ab249319)](https://www.abcam.com/ps/products/249/ab249319/Images/ab249319-408086-anti-adrm1-arm-1-antibody-epr11450-b-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-ADRM1/ARM-1 antibody [EPR11450(B)] - BSA and Azide free (ab249319)All lanes : Anti-ADRM1/ARM-1 antibody [EPR11450(B)] (ab157218) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : RAW 264.7 cell lysate
Lane 4 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 42 kDaThis data was developed using ab157218, the same antibody clone in a different buffer formulation.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRM1/ARM-1 antibody [EPR11450(B)] - BSA and Azide free (ab249319)](https://www.abcam.com/ps/products/249/ab249319/Images/ab249319-2-anti-adrm1-arm-1-antibody-epr11450-b-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRM1/ARM-1 antibody [EPR11450(B)] - BSA and Azide free (ab249319)This data was developed using ab157218, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue labeling ADRM1/ARM-1 with ab157218 at 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Flow Cytometry - Anti-ADRM1/ARM-1 antibody [EPR11450(B)] - BSA and Azide free (ab249319)](https://www.abcam.com/ps/products/249/ab249319/Images/ab249319-1-anti-adrm1-arm-1-antibody-epr11450-b-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-ADRM1/ARM-1 antibody [EPR11450(B)] - BSA and Azide free (ab249319) -
![Anti-ADRM1/ARM-1 antibody [EPR11450(B)] - BSA and Azide free (ab249319)](https://www.abcam.com/ps/products/249/ab249319/Images/ab249319-6-benefits-of-recombinant-antibodies.png)
Anti-ADRM1/ARM-1 antibody [EPR11450(B)] - BSA and Azide free (ab249319)