Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Histone H3 (acetyl K64) antibody [EPR20713] - BSA and Azide free (ab251549)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20713] to Histone H3 (acetyl K64) - BSA and Azide free
  • Suitable for: PepArr, ICC, ChIP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Histone H3 (acetyl K64) antibody [EPR20713] - BSA and Azide free
    See all Histone H3 primary antibodies

  • Description

    Rabbit monoclonal [EPR20713] to Histone H3 (acetyl K64) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ChIP
    Human
    PepArr
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251549 is the carrier-free version of ab214808. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251549 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Clonality

    Monoclonal

  • Clone number

    EPR20713

  • Isotype

    IgG

Images

  • ChIP - Anti-Histone H3 (acetyl K64) antibody [EPR20713] - BSA and Azide free (ab251549)
    ChIP - Anti-Histone H3 (acetyl K64) antibody [EPR20713] - BSA and Azide free (ab251549)

    This data was developed using ab214808, the same antibody clone in a different buffer formulation.

    Chromatin was prepared from HeLa (human epithelial cell line from cervix adenocarcinoma) cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab214808(red), and 20µl of Anti rabbit IgG sepharose beads. 2μg of rabbit normal IgG was added to the beads control (grey). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.
  • Immunocytochemistry - Anti-Histone H3 (acetyl K64) antibody [EPR20713] - BSA and Azide free (ab251549)
    Immunocytochemistry - Anti-Histone H3 (acetyl K64) antibody [EPR20713] - BSA and Azide free (ab251549)
    This data was developed using ab214808, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 100% methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells, untreated and treated with Trichostatin A (500 ng/ml) for 4 hours, labeling Histone H3 (acetyl K64) with ab214808 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cells. The expression increased after treatment with Trichostatin A (500 ng/ml) for 4 hours. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
  • Western blot - Anti-Histone H3 (acetyl K64) antibody [EPR20713] - BSA and Azide free (ab251549)
    Western blot - Anti-Histone H3 (acetyl K64) antibody [EPR20713] - BSA and Azide free (ab251549)
    All lanes : Anti-Histone H3 (acetyl K64) antibody [EPR20713] - ChIP Grade (ab214808) at 1/5000 dilution

    Lane 1 : Untreated NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 2 : NIH/3T3 treated with 500ng/ml Trichostatin A for 4 hours whole cell lysate
    Lane 3 : Untreated C6 (rat glial tumor cell line) whole cell lysate
    Lane 4 : C6 treated with 500ng/ml Trichostatin A for 4 hours whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 15 kDa
    Observed band size: 15 kDa


    Exposure time: 3 minutes


    This data was developed using ab214808, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Histone H3 (acetyl K64) antibody [EPR20713] - BSA and Azide free (ab251549)
    Western blot - Anti-Histone H3 (acetyl K64) antibody [EPR20713] - BSA and Azide free (ab251549)
    All lanes : Anti-Histone H3 (acetyl K64) antibody [EPR20713] - ChIP Grade (ab214808) at 1/1000 dilution

    Lane 1 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HeLa treated with 400ng/ml Trichostatin A for 9 hours whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 15 kDa
    Observed band size: 15 kDa


    Exposure time: 15 seconds


    This data was developed using ab214808, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Peptide Array - Anti-Histone H3 (acetyl K64) antibody [EPR20713] - BSA and Azide free (ab251549)
    Peptide Array - Anti-Histone H3 (acetyl K64) antibody [EPR20713] - BSA and Azide free (ab251549)
    This data was developed using ab214808, the same antibody clone in a different buffer formulation.ab214808 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate). Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity. The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.
  • Anti-Histone H3 (acetyl K64) antibody [EPR20713] - BSA and Azide free (ab251549)
    Anti-Histone H3 (acetyl K64) antibody [EPR20713] - BSA and Azide free (ab251549)

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