Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Argininosuccinate Lyase antibody [EPR19382] - BSA and Azide free (ab251330)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19382] to Argininosuccinate Lyase - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Argininosuccinate Lyase antibody [EPR19382] - BSA and Azide free
    See all Argininosuccinate Lyase primary antibodies

  • Description

    Rabbit monoclonal [EPR19382] to Argininosuccinate Lyase - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251330 is the carrier-free version of ab201025. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251330 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Argininosuccinate Lyase antibody [EPR19382] - BSA and Azide free (ab251330)
    Western blot - Anti-Argininosuccinate Lyase antibody [EPR19382] - BSA and Azide free (ab251330)
    All lanes : Anti-Argininosuccinate Lyase antibody [EPR19382] (ab201025) at 1/1000 dilution

    Lane 1 : Human fetal heart tissue lysate
    Lane 2 : Human fetal kidney tissue lysate
    Lane 3 : Human fetal spleen tissue lysate
    Lane 4 : Human fetal liver tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

    Predicted band size: 51 kDa
    Observed band size: 51 kDa



    This data was developed using ab201025, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: Lanes 1-3: 8 seconds; Lane 4: 1 second.

  • Western blot - Anti-Argininosuccinate Lyase antibody [EPR19382] - BSA and Azide free (ab251330)
    Western blot - Anti-Argininosuccinate Lyase antibody [EPR19382] - BSA and Azide free (ab251330)
    All lanes : Anti-Argininosuccinate Lyase antibody [EPR19382] (ab201025) at 1/1000 dilution

    Lane 1 : Untreated HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) treated with 1 µM dexamethasone for 24 hours whole cell lysate
    Lane 3 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 5 : T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate
    Lane 6 : A549 (Human lung carcinoma cell line) whole cell lysate
    Lane 7 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 51 kDa
    Observed band size: 51 kDa



    This data was developed using ab201025, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: Lanes 1-2: 1 second; Lanes 3-5: 15 seconds; Lanes 6-7: 30 seconds.

    Dexamethasone treatment increase the accumulation of ASL mRNA (PMID: 2209616).

  • Immunoprecipitation - Anti-Argininosuccinate Lyase antibody [EPR19382] - BSA and Azide free (ab251330)
    Immunoprecipitation - Anti-Argininosuccinate Lyase antibody [EPR19382] - BSA and Azide free (ab251330)
    This data was developed using ab201025, the same antibody clone in a different buffer formulation.Argininosuccinate Lyase was immunoprecipitated from 1mg of Human fetal liver whole cell lysate with ab201025 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab201025 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: Human fetal liver whole cell lysate, 10µg (Input). Lane 2: ab201025 IP in Human fetal liver whole cell lysate. Lane 3: Rabbit IgG,monoclonal-Isotype Control (ab172730) instead of ab201025 in Human fetal liver whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 5 seconds.
  • Anti-Argininosuccinate Lyase antibody [EPR19382] - BSA and Azide free (ab251330)
    Anti-Argininosuccinate Lyase antibody [EPR19382] - BSA and Azide free (ab251330)

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