Anti-PLA2R antibody [12-6-5] (ab211490)
![Anti-PLA2R antibody [12-6-5] (ab211490)](https://www.abcam.com/ps/products/211/ab211490/Images/ab211490-259688-anti-pla2r-antibody-12-6-5-western-blot.jpg "Anti-PLA2R antibody [12-6-5] (ab211490)")
Key features and details
- Mouse monoclonal [12-6-5] to PLA2R
- Suitable for: WB, Flow Cyt, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-PLA2R antibody [12-6-5]
See all PLA2R primary antibodies -
Description
Mouse monoclonal [12-6-5] to PLA2R -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human PLA2R aa 1-700. corresponding to extracellular sequence NC3 (containing domains N-terminal Cysteine rich-FibII-CTLD1-CTLD2-CTLD3) expressed in HEK 293-EBNA-1 cells
Database link: Q13018 -
Positive control
- WB: Recombinant extracellular domain (NC8) and N terminal fragment (NC3; immunogen) of human PLA2R. ICC/IF: PLA2R over-expressing podocytes. IHC-P: Normal human kidney and membranous nephropathy kidney tissues. Flow Cytometry: PLA2R over-expressing podocytes.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Dissociation constant (KD)
KD = 5.40 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Sterilization by membrane filtration. -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
12-6-5 -
Myeloma
Sp2 -
Isotype
IgG2b -
Research areas
Images
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Western blot - Anti-PLA2R antibody [12-6-5] (ab211490)All lanes : Anti-PLA2R antibody [12-6-5] (ab211490) at 1/10000 dilution
Lane 1 : Recombinant extracellular domain of human PLA2R (NC8)
Lane 2 : Recombinant N-terminus fragment of human PLA2R (NC3)
Lysates/proteins at 10 µg per lane.
Predicted band size: 169 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLA2R antibody [12-6-5] (ab211490)](https://www.abcam.com/ps/products/211/ab211490/Images/ab211490-259693-anti-pla2r-antibody-12-6-5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLA2R antibody [12-6-5] (ab211490)Immunohistochemical analysis of membranous nephropathy kidney tissue labeling PLA2R at 1/5000 dilution followed by DAB detection.
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![Immunocytochemistry/ Immunofluorescence - Anti-PLA2R antibody [12-6-5] (ab211490)](https://www.abcam.com/ps/products/211/ab211490/Images/ab211490-259690-anti-pla2r-antibody-12-6-5-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PLA2R antibody [12-6-5] (ab211490)Immunofluorescent analysis of PLA2R over-expressing podocytes labeling PLA2R at 1/400 dilution (green).
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![Flow Cytometry - Anti-PLA2R antibody [12-6-5] (ab211490)](https://www.abcam.com/ps/products/211/ab211490/Images/ab211490-259689-anti-pla2r-antibody-12-6-5-flow-cytometry.jpg)
Flow Cytometry - Anti-PLA2R antibody [12-6-5] (ab211490)Flow cytometry analysis of wild type (dark blue line) and over-expressing PLA2R podocytes (low expressor, green line; high expressor, red line) labeling PLA2R using ab211490 at 1/200 dilution. Negative controls were unlabelled cells (turquoise line) and mouse IgG (grey line).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLA2R antibody [12-6-5] (ab211490)](https://www.abcam.com/ps/products/211/ab211490/Images/ab211490-259692-anti-pla2r-antibody-12-6-5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLA2R antibody [12-6-5] (ab211490)Immunohistochemical analysis of normal human kidney tissue labeling PLA2R at 1/5000 dilution followed by DAB detection.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLA2R antibody [12-6-5] (ab211490)](https://www.abcam.com/ps/products/211/ab211490/Images/ab211490-259691-anti-pla2r-antibody-12-6-5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLA2R antibody [12-6-5] (ab211490)Immunohistochemical analysis of normal human kidney tissue labeling PLA2R at 1/5000 dilution followed by DAB detection.
