Anti-AGO3 antibody [EPR9576] (ab154844)
![Anti-AGO3 antibody [EPR9576] (ab154844)](https://www.abcam.com/ps/products/154/ab154844/Images/ab154844-387258-anti-ago3-antibody-epr9576-western-blot-hela-daudi-a549-lysates.jpg "Anti-AGO3 antibody [EPR9576] (ab154844)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9576] to AGO3
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-AGO3 antibody [EPR9576]
See all AGO3 primary antibodies -
Description
Rabbit monoclonal [EPR9576] to AGO3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
corresponding to Human AGO3.
Database link: Q9H9G7 -
Positive control
- WB: HeLa, Daudi, A549 cell lysates. Human fetal brain and fetal kidney lysates.
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General notes
This product was previously labelled as Argonaute 3 / eIF2C 3
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9576 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-AGO3 antibody [EPR9576] (ab154844)All lanes : Anti-AGO3 antibody [EPR9576] (ab154844) at 1/2000 dilution
Lane 1 : Wild-type HeLa lysate
Lane 2 : Argonaute RISC Catalytic Component 3 knockout HeLa lysate
Lane 3 : Daudi lysate
Lane 4 : A549 lysate
Lysates/proteins at 40 µg per lane.
Performed under reducing conditions.
Predicted band size: 97 kDaLanes 1-4: Merged signal (red and green). Green - ab154844 observed at 97 kDa. Red - loading control ab8245 observed at 37 kDa.
ab154844 Anti-AGO3 antibody [EPR9576] was shown to specifically react with Argonaute RISC Catalytic Component 3 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265320 (knockout cell lysate ab257819) was used. Wild-type and Argonaute RISC Catalytic Component 3 knockout samples were subjected to SDS-PAGE. ab154844 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 2000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-AGO3 antibody [EPR9576] (ab154844)](https://www.abcam.com/ps/products/154/ab154844/Images/ab154844-161444-anti-argonaute-3-eif2c-3-antibody-epr9576-western-blot.jpg)
Western blot - Anti-AGO3 antibody [EPR9576] (ab154844)All lanes : Anti-AGO3 antibody [EPR9576] (ab154844) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal kidney lysate
Lysates/proteins at 10 µl/ml buffer per lane.
Predicted band size: 97 kDa
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![Anti-AGO3 antibody [EPR9576] (ab154844)](https://www.abcam.com/ps/products/154/ab154844/Images/ab154844-4-benefits-of-recombinant-antibodies.png)
Anti-AGO3 antibody [EPR9576] (ab154844)
