Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PLA2R antibody [EPR20483] (ab211573)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20483] to PLA2R
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-PLA2R antibody [EPR20483]
    See all PLA2R primary antibodies

  • Description

    Rabbit monoclonal [EPR20483] to PLA2R

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal kidney lysate. IHC-P: Human kidney tissue (from a patient with renal clear cell carcinoma).

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLA2R antibody [EPR20483] (ab211573)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLA2R antibody [EPR20483] (ab211573)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue (from a patient with renal clear cell carcinoma) labeling PLA2R with ab211573 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Positive staining on glomerular basement membrane of human kidney (PMID: 26222864).

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-PLA2R antibody [EPR20483] (ab211573)
    Western blot - Anti-PLA2R antibody [EPR20483] (ab211573)
    All lanes : Anti-PLA2R antibody [EPR20483] (ab211573) at 1/1000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal liver lysate
    Lane 3 : Human fetal heart lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 169 kDa
    Observed band size: 180 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    PLA2R is specifically expressed in kidney (PMID: 7721806).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLA2R antibody [EPR20483] (ab211573)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLA2R antibody [EPR20483] (ab211573)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling PLA2R with ab211573 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Negative control: No staining on human pancreas (PMID: 7721806).

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-PLA2R antibody [EPR20483] (ab211573)
    Anti-PLA2R antibody [EPR20483] (ab211573)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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