Anti-BCAR3/NSP2 antibody (ab85940)
Key features and details
- Rabbit polyclonal to BCAR3/NSP2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-BCAR3/NSP2 antibody
See all BCAR3/NSP2 primary antibodies -
Description
Rabbit polyclonal to BCAR3/NSP2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human -
Immunogen
Synthetic peptide corresponding to Human BCAR3/NSP2 aa 25-75.
Database link: NP_003558.1 -
Positive control
- HeLa and 293T whole cell lysates
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General notes
This product was previously labelled as BCAR3
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab85940 was affinity purified using an epitope specific to BCAR3/NSP2 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-BCAR3/NSP2 antibody (ab85940) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 93 kDa
Observed band size: 93 kDa
Additional bands at: 300 kDa, 460 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
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Detection of human BCAR3/NSP2 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85940 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot.
Detection: Chemiluminescence with an exposure time of 10 seconds.