Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HLA A2 antibody [BB7.2] (ab74674)

Key features and details

  • Mouse monoclonal [BB7.2] to HLA A2 - BSA and Azide free
  • Suitable for: IP, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG2b

Overview

  • Product name

    Anti-HLA A2 antibody [BB7.2] - BSA and Azide free
    See all HLA A2 primary antibodies

  • Description

    Mouse monoclonal [BB7.2] to HLA A2 - BSA and Azide free

  • Host species

    Mouse

  • Specificity

    This product has been purified from tissue culture supernatant.

  • Tested applications

    Suitable for: IP, Flow Cytmore details
    Unsuitable for: WB

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Full length protein corresponding to Human HLA A2. HLA-A2 solubilised by papain Full length Native protein

  • Epitope

    The C-terminus of alpha-2 helix and a turn on one of the underlying beta strands.

  • General notes

    This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.40
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    This product has been purified from tissue culture supernatant. Purity >95% by SDS-PAGE.

  • Clonality

    Monoclonal

  • Clone number

    BB7.2

  • Isotype

    IgG2b

  • Light chain type

    kappa

  • Research areas

Images

  • Flow Cytometry - Anti-HLA A2 antibody [BB7.2] (ab74674)
    Flow Cytometry - Anti-HLA A2 antibody [BB7.2] (ab74674)
    Flow cytometry of human peripheral blood cells with ab74674 at 2 µg/ml
  • Flow Cytometry - Anti-HLA A2 antibody [BB7.2] (ab74674)
    Flow Cytometry - Anti-HLA A2 antibody [BB7.2] (ab74674)
    Overlay histogram showing Raji cells stained with ab74674 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74674, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed Raji cells used under the same conditions.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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