Anti-TRRAP antibody - N-terminal (ab227613)
Key features and details
- Rabbit polyclonal to TRRAP - N-terminal
- Suitable for: WB, ICC/IF, IP, ChIP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TRRAP antibody - N-terminal
See all TRRAP primary antibodies -
Description
Rabbit polyclonal to TRRAP - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IP, ChIPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
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Immunogen
Recombinant fragment within Human TRRAP (N terminal). The exact sequence is proprietary.
Database link: Q9Y4A5 -
Positive control
- WB: Jurkat, Raji, NCI-H929, HEK-293T, A431, HeLa and HepG2 whole cell lysates. IP: HEK-293T whole cell extract. ChIP: HeLa chromatin extract. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-TRRAP antibody - N-terminal (ab227613)
TRRAP was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 4 μg ab227613. Western blot was performed from the immunoprecipitate using ab227613 at 1/500 dilution. Anti-rabbit IgG was used as secondary antibody.
Lane 1: Control IP in HEK-293T whole cell extract with 4 μg of preimmune Rabbit IgG.
Lane 2: ab227613 IP in HEK-293T whole cell extract.
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ChIP - Anti-TRRAP antibody - N-terminal (ab227613)Cross-linked ChIP was performed with HeLa (human epithelial cell line from cervix adenocarcinoma) chromatin extract and 5 μg of either control rabbit IgG or ab227613. The precipitated DNA was detected by PCR with primer set targeting to nucleolin promoter.
ChIP experiment and primer designs are based on EMBO J. 2007 Jul 25;26(14):3332-45.
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Immunocytochemistry/ Immunofluorescence - Anti-TRRAP antibody - N-terminal (ab227613)4% paraformaldehyde fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for TRRAP (green) using ab227613 at 1/1000 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
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Western blot - Anti-TRRAP antibody - N-terminal (ab227613)All lanes : Anti-TRRAP antibody - N-terminal (ab227613) at 1/500 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : NCI-H929 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 438 kDa5 % SDS-PAGE gel.
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Western blot - Anti-TRRAP antibody - N-terminal (ab227613)All lanes : Anti-TRRAP antibody - N-terminal (ab227613) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 438 kDa5 % SDS-PAGE gel.
