Anti-TRRAP antibody - ChIP Grade (ab73546)
Key features and details
- Rabbit polyclonal to TRRAP - ChIP Grade
- Suitable for: WB, ChIP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TRRAP antibody - ChIP Grade
See all TRRAP primary antibodies -
Description
Rabbit polyclonal to TRRAP - ChIP Grade -
Host species
Rabbit -
Tested applications
Suitable for: WB, ChIPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Xenopus laevis
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Immunogen
Synthetic peptide corresponding to Human TRRAP aa 3800 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab101312) -
Positive control
- This antibody gave a positive signal in the following nuclear lysate preparations: HeLa; Jurkat.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab73546 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 438 kDa (predicted molecular weight: 438 kDa). ChIP Use 5 µg for 25 µg of chromatin. Target
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Relevance
TRRAP (TRansformation/tRanscription domain Associated Protein) is an adapter protein that is found in a number of multiprotein chromatin complexes with histone acetyltransferase (HAT) activity. These complexes include PCAF, TFTC-HAT, TIP60 HAT, STAGA, and BAF53 complexes. TRRAP is thought to be responsible for the concerted and context-dependent recruitment of these complexes to chromatin during transcription, replication and DNA repair. TRRAP plays a key role in transcriptional activation by c-Myc, p53/TP53, E2F1 and E2F4. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 8295 Human
- Entrez Gene: 100683 Mouse
- Entrez Gene: 288471 Rat
- Omim: 603015 Human
- SwissProt: Q9Y4A5 Human
- SwissProt: Q80YV3 Mouse
- Unigene: 203952 Human
- Unigene: 273570 Mouse
see all -
Alternative names
- 350/400 kDa PCAF associated factor antibody
- PAF 350/400 antibody
- PAF 400 antibody
see all
Images
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Western blot - Anti-TRRAP antibody - ChIP Grade (ab73546)All lanes : Anti-TRRAP antibody - ChIP Grade (ab73546) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 2 : Jurkat nuclear extract lysate (ab14844)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 438 kDa
Observed band size: 438 kDa
Additional bands at: 150 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
ChIP - Anti-TRRAP antibody - ChIP Grade (ab73546)Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 5µg of ab73546 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach for both the active and heterochromatic loci). Primers are located in the first kb of the transcribed region.
Datasheets and documents
References (4)
ab73546 has been referenced in 4 publications.
- Sheng X et al. IRE1a-XBP1s pathway promotes prostate cancer by activating c-MYC signaling. Nat Commun 10:323 (2019). PubMed: 30679434
- Wang Z et al. TRRAP is a central regulator of human multiciliated cell formation. J Cell Biol 217:1941-1955 (2018). PubMed: 29588376
- Han SI et al. Estrogen receptor ligands ameliorate fatty liver through a nonclassical estrogen receptor/Liver X receptor pathway in mice. Hepatology 59:1791-802 (2014). PubMed: 24277692
- O'Neill DJ et al. SETD6 controls the expression of estrogen-responsive genes and proliferation of breast carcinoma cells. Epigenetics 9:942-50 (2014). PubMed: 24751716
Images
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Western blot - Anti-TRRAP antibody - ChIP Grade (ab73546)All lanes : Anti-TRRAP antibody - ChIP Grade (ab73546) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 2 : Jurkat nuclear extract lysate (ab14844)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 438 kDa
Observed band size: 438 kDa
Additional bands at: 150 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
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ChIP - Anti-TRRAP antibody - ChIP Grade (ab73546)Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 5µg of ab73546 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach for both the active and heterochromatic loci). Primers are located in the first kb of the transcribed region.
