Anti-TRRAP antibody (ab227590)
Key features and details
- Rabbit polyclonal to TRRAP
- Suitable for: IP, ChIP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TRRAP antibody
See all TRRAP primary antibodies -
Description
Rabbit polyclonal to TRRAP -
Host species
Rabbit -
Tested applications
Suitable for: IP, ChIP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment within Human TRRAP (internal sequence). The exact sequence is proprietary.
Database link: Q9Y4A5 -
Positive control
- ChIP: HeLa chromatin extract. WB: Jurkat, Raji, NCI-H929, HEK-293T, A431, HeLa and HepG2 whole cell lysates. IP: HEK-293T whole cell extract.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Cross-linked ChIP was performed with HeLa (human epithelial cell line from cervix adenocarcinoma) chromatin extract and 5 μg of either control rabbit IgG or ab227590. The precipitated DNA was detected by PCR with primer set targeting to nucleolin promoter.
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All lanes : Anti-TRRAP antibody (ab227590) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 438 kDa5% SDS-PAGE gel.
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TRRAP was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 4 µg of ab227590. Western blot was performed from the immunoprecipitate using ab227590 at 1/1000 dilution. Anti-rabbit IgG was used as a secondary reagent.
Lane 1: 4 μg of preimmune Rabbit IgG instead of ab227590 in HEK-293T whole cell extract.
Lane 2: ab227590 IP in HEK-293T whole cell extract.5% SDS-PAGE gel.
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All lanes : Anti-TRRAP antibody (ab227590) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : NCI-H929 (human bone marrow lymphoblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 438 kDa5% SDS-PAGE gel.