Antibodies, Proteins, Kits and Reagents for Life Science

Anti-TRIM44 antibody [OTI1E10] (ab236422)

Key features and details

Mouse monoclonal [OTI1E10] to TRIM44
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Human
Isotype: IgG1

Product name

Anti-TRIM44 antibody [OTI1E10]
See all TRIM44 primary antibodies
Description

Mouse monoclonal [OTI1E10] to TRIM44
Host species

Mouse
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

Recombinant fragment

See all applications and species data
Immunogen

Recombinant full length protein corresponding to Human TRIM44 aa 1 to the C-terminus. (NP_060053) produced in HEK-293T cells.
Database link: Q96DX7

Run BLAST with Run BLAST with
Positive control
- WB: HEK-293T transfected with pCMV6-ENTRY TRIM44, whole cell lysate. IHC-P: Human breast, kidney, liver, liver carcinoma, pancreas, endometrium, bladder carcinoma, lymphoma and tonsil tissue. ICC/IF: COS-7 cells transiently transfected with pCMV6-ENTRY TRIM44.
General notes

Clone OTI1E10 (formerly 1E10).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
IHC-P	Human
WB	Human Recombinant fragment

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 50% Glycerol, PBS
Concentration information loading...
Purity

Affinity purified
Purification notes

Purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

OTI1E10
Isotype

IgG1
Research areas

Western blot - Anti-TRIM44 antibody [OTI1E10] (ab236422)

All lanes : Anti-TRIM44 antibody [OTI1E10] (ab236422) at 1/2000 dilution

Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY control cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY TRIM44 cell lysate

Lysates/proteins at 5 µg per lane.

Predicted band size: 38 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM44 antibody [OTI1E10] (ab236422)

Paraffin-embedded human breast tissue stained for TRIM44 using ab236422 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM44 antibody [OTI1E10] (ab236422)

Paraffin-embedded human kidney tissue stained for TRIM44 using ab236422 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM44 antibody [OTI1E10] (ab236422)

Paraffin-embedded human liver tissue stained for TRIM44 using ab236422 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM44 antibody [OTI1E10] (ab236422)

Paraffin-embedded human liver cancer tissue stained for TRIM44 using ab236422 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM44 antibody [OTI1E10] (ab236422)

Paraffin-embedded human pancreas tissue stained for TRIM44 using ab236422 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM44 antibody [OTI1E10] (ab236422)

Paraffin-embedded human endometrium tissue stained for TRIM44 using ab236422 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM44 antibody [OTI1E10] (ab236422)

Paraffin-embedded human bladder carcinoma tissue stained for TRIM44 using ab236422 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM44 antibody [OTI1E10] (ab236422)

Paraffin-embedded human lymphoma tissue stained for TRIM44 using ab236422 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM44 antibody [OTI1E10] (ab236422)

Paraffin-embedded human tonsil tissue stained for TRIM44 using ab236422 at 1/150 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-TRIM44 antibody [OTI1E10] (ab236422)

COS-7 (human epithelial cell line from cervix adenocarcinoma) cells transiently transfected with pCMV6-ENTRY TRIM44 stained for TRIM44 (green) using ab236422 at 1/100 dilution in ICC/IF.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TRIM44 antibody [OTI1E10] (ab236422)

Key features and details

Overview

Product name

Description

Host species

Tested Applications & Species

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Purification notes

Clonality

Clone number

Isotype

Research areas

Images

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