All lanes : Anti-CDKL5 antibody (ab224052) at 0.4 µg/ml

Lane 1 : Control (vector only transfected) - HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : CDKL5 over-expression - HEK-293T whole cell lysate, Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)

Predicted band size: 116 kDa

Immunohistochemical analysis of human testis tissue labeling CDKL5 in the nucleus of cells with ab224052 at 1/200 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunofluorescent analysis of A431 (human epidermoid carcinoma cell line) cells labeling CDKL5 (green) in the nucleoplasm with ab224052 at 2 µg/ml.

Immunohistochemical analysis of human placenta tissue labeling CDKL5 in the nucleus of trophoblastic cells with ab224052 at 1/200 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemical analysis of human lung tissue labeling CDKL5 in the nucleus of macrophages cells with ab224052 at 1/200 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemical analysis of human skeletal mucle tissue labeling CDKL5 with ab224052 at 1/200 dilution. No positivity as expected.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.