Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-KMT2B / MLL4 antibody [4C10] (ab56770)

Key features and details

  • Mouse monoclonal [4C10] to KMT2B / MLL4
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG1

Overview

  • Product name

    Anti-KMT2B / MLL4 antibody [4C10]
    See all KMT2B / MLL4 primary antibodies

  • Description

    Mouse monoclonal [4C10] to KMT2B / MLL4

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Human, Recombinant fragment

  • Immunogen

    Recombinant fragment (proprietary-tag) corresponding to Human KMT2B/ MLL4 aa 813-904. Entrez gene ID: 9757
    Sequence:

    KVAASMPLSPGGQMEEVAGAVKQISDRGPVRSEDESVEAKRERPSGPESP VQGPRIKHVCRHAAVALGQARAMVPEDVPRLSALPLRDRQDL


    Database link: Q9UMN6
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • General notes

    This product was changed from ascites to tissue culture supernatant on 11th June 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Images

  • Western blot - Anti-KMT2B / MLL4 antibody [4C10] (ab56770)
    Western blot - Anti-KMT2B / MLL4 antibody [4C10] (ab56770)

    Western blot against tagged recombinant protein immunogen using ab56770 MLL4 antibody at 1ug/ml. Predicted band size of immunogen is 36 kDa

    This image was generated using the ascites version of the product.

  • Flow Cytometry - Anti-KMT2B / MLL4 antibody [4C10] (ab56770)
    Flow Cytometry - Anti-KMT2B / MLL4 antibody [4C10] (ab56770)

    Overlay histogram showing HL60 cells stained with ab56770 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56770, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    This image was generated using the ascites version of the product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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