Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MHC class I + HLA B antibody [EP2624] (ab110645)

Key features and details

  • Rabbit monoclonal [EP2624] to MHC class I + HLA B
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-MHC class I + HLA B antibody [EP2624]

  • Description

    Rabbit monoclonal [EP2624] to MHC class I + HLA B

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Ramos, HL60, Jurkat and TF1 cell lysates Paraffin embedded Human tonsils IP: Ramos whole cell lysate.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EP2624

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-MHC class I + HLA B antibody [EP2624] (ab110645)
    Western blot - Anti-MHC class I + HLA B antibody [EP2624] (ab110645)
    All lanes : Anti-MHC class I + HLA B antibody [EP2624] (ab110645) at 1/1000 dilution

    Lane 1 : Ramos cell lysate
    Lane 2 : HL 60 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : TF1 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size: 40 kDa

  • Immunoprecipitation - Anti-MHC class I + HLA B antibody [EP2624] (ab110645)
    Immunoprecipitation - Anti-MHC class I + HLA B antibody [EP2624] (ab110645)

    Purified ab110645 at 1/50 dilution (2µg) immunoprecipitating MHC class I+HLA B in Ramos whole cell lysate.
    Lane 1 (input): Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10µg
    Lane 2 (+): ab110645 + Ramos whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab110645 in Ramos whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 40 kDa
    ?2m could also be observed at around 15kDa.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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