Anti-MHC class I + HLA B antibody [EP2624] (ab110645)
![Anti-MHC class I + HLA B antibody [EP2624] (ab110645)](https://www.abcam.com/ps/products/110/ab110645/Images/ab110645-123960-anti-mhc-class-i-hla-b-antibody-ep2624-western-blot.jpg "Anti-MHC class I + HLA B antibody [EP2624] (ab110645)")
Key features and details
- Rabbit monoclonal [EP2624] to MHC class I + HLA B
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MHC class I + HLA B antibody [EP2624] -
Description
Rabbit monoclonal [EP2624] to MHC class I + HLA B -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Ramos, HL60, Jurkat and TF1 cell lysates Paraffin embedded Human tonsils IP: Ramos whole cell lysate.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP2624 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MHC class I + HLA B antibody [EP2624] (ab110645)All lanes : Anti-MHC class I + HLA B antibody [EP2624] (ab110645) at 1/1000 dilution
Lane 1 : Ramos cell lysate
Lane 2 : HL 60 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : TF1 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 40 kDa
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![Immunoprecipitation - Anti-MHC class I + HLA B antibody [EP2624] (ab110645)](https://www.abcam.com/ps/products/110/ab110645/Images/ab110645-3-anti-mhc-class-I-hla-b-antibody-ep2624-immunoprecipitation-ramos-human.jpg)
Immunoprecipitation - Anti-MHC class I + HLA B antibody [EP2624] (ab110645)Purified ab110645 at 1/50 dilution (2µg) immunoprecipitating MHC class I+HLA B in Ramos whole cell lysate.
Lane 1 (input): Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10µg
Lane 2 (+): ab110645 + Ramos whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab110645 in Ramos whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 40 kDa
?2m could also be observed at around 15kDa.
