Anti-PRKAR1A (phospho S83) antibody [EPMAYR1-111] (ab154851)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPMAYR1-111] to PRKAR1A (phospho S83)
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
Overview
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Product name
Anti-PRKAR1A (phospho S83) antibody [EPMAYR1-111]
See all PRKAR1A primary antibodies -
Description
Rabbit monoclonal [EPMAYR1-111] to PRKAR1A (phospho S83) -
Host species
Rabbit -
Specificity
ab154851 only detects Protein Kinase A regulatory subunit I alpha/PRKAR1A phosphorylated at serine 83.
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Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Rat -
Immunogen
Synthetic peptide corresponding to Human PRKAR1A.
Database link: P10644 -
Positive control
- Rat heart lysate; Human kidney and prostate tissues.
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General notes
This antibody was developed as part of a collaboration between Abcam and the lab of Dr. Ozgur Ogut at the Mayo Clinic.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as Protein Kinase A regulatory subunit I alpha
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPMAYR1-111 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-PRKAR1A (phospho S83) antibody [EPMAYR1-111] (ab154851) at 1/1000 dilution
Lane 1 : Rat heart lysate; untreated
Lane 2 : Rat heart lysate; treated with alkaline phosphatase
Lysates/proteins at 10 µg per lane.
Predicted band size: 43 kDaLower panel shows labeling with a non-phospho Protein Kinase A regulatory subunit I alpha/PRKAR1A antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR1A (phospho S83) antibody [EPMAYR1-111] (ab154851)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Protein Kinase A regulatory subunit I alpha/PRKA1A with ab154851 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR1A (phospho S83) antibody [EPMAYR1-111] (ab154851)
Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling Protein Kinase A regulatory subunit I alpha/PRKAR1A with ab154851 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR1A (phospho S83) antibody [EPMAYR1-111] (ab154851)
Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154851 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR1A (phospho S83) antibody [EPMAYR1-111] (ab154851)
Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue using ab154851 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR1A (phospho S83) antibody [EPMAYR1-111] (ab154851)
Immunohistochemical analysis of paraffin embedded Human normal liver tissue using ab154851 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR1A (phospho S83) antibody [EPMAYR1-111] (ab154851)
Immunohistochemical analysis of paraffin embedded Human normal brain tissue using ab154851 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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