Anti-PRKAR1A antibody - C-terminal (ab230714)
Images
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Western blot - Anti-PRKAR1A antibody - C-terminal (ab230714)All lanes : Anti-PRKAR1A antibody - C-terminal (ab230714) at 1/500 dilution
Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate
Lane 3 : NCI-H1299 (Human lung carcinoma cell line) whole cell lysate
Lane 4 : A549 (human lung carcinoma cell line) whole cell lysate
Predicted band size: 43 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR1A antibody - C-terminal (ab230714)Formalin-fixed, paraffin-embedded human testis tissue stained for Protein Kinase A regulatory subunit I alpha/PRKAR1A using ab230714 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-PRKAR1A antibody - C-terminal (ab230714)Formalin-fixed, 0.1% Triton X-100 permeabilized HT-29 (Human colorectal adenocarcinoma cell line) cells stained for Anti-Protein Kinase A regulatory subunit I alpha/PRKAR1A using ab230714 at 1/100 dilution in ICC/IF.
Cells blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
