Anti-PRKAR1A (phospho S77) antibody [EPMAYR1-127] (ab139682)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPMAYR1-127] to PRKAR1A (phospho S77)
- Suitable for: WB
- Reacts with: Human
Overview
-
Product name
Anti-PRKAR1A (phospho S77) antibody [EPMAYR1-127]
See all PRKAR1A primary antibodies -
Description
Rabbit monoclonal [EPMAYR1-127] to PRKAR1A (phospho S77) -
Host species
Rabbit -
Specificity
ab139682 only detects Protein Kinase A regulatory subunit I alpha/PRKAR1A phosphorylated at serine 77.
-
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Synthetic peptide corresponding to Human PRKAR1A.
-
Positive control
- Fetal heart tissue lysate.
-
General notes
This antibody was developed as part of a collaboration between Abcam and the lab of Dr. Ozgur Ogut at the Mayo Clinic.
This product was previously labelled as Protein Kinase A regulatory subunit I alpha
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPMAYR1-127 -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-PRKAR1A (phospho S77) antibody [EPMAYR1-127] (ab139682) at 1/1000 dilution
Lane 1 : Fetal heart lysate
Lane 2 : Fetal heart lysate treated with Alkaline Phosphatase.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 43 kDa
-