Anti-CDK1 (phospho Y15) antibody [BLR101H] (ab275958)
![Anti-CDK1 (phospho Y15) antibody [BLR101H] (ab275958)](https://www.abcam.com/ps/products/275/ab275958/Images/ab275958-411886-AntiCDK1-antibody-BLR101H-westernblot-HeLa-cells-human.JPG "Anti-CDK1 (phospho Y15) antibody [BLR101H] (ab275958)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR101H] to CDK1 (phospho Y15)
- Suitable for: WB, IP
- Reacts with: Human, Recombinant fragment
Overview
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Product name
Anti-CDK1 (phospho Y15) antibody [BLR101H]
See all CDK1 primary antibodies -
Description
Rabbit monoclonal [BLR101H] to CDK1 (phospho Y15) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Recombinant fragment
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Immunogen
Synthetic peptide corresponding to Human CDK1 (phospho Y15). NP_001777.1
Database link: P06493 -
Positive control
- WB: HeLa cells treated with Hydroxyurea. IP: HeLa cells treated with Hydroxyurea.
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General notes
This product is sold under License from Bethyl Laboratories, Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.20
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Borate buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
BLR101H -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CDK1 antibody [BLR101H] (ab275958)All lanes : Anti-CDK1 (phospho Y15) antibody [BLR101H] (ab275958) at 1/1000 dilution
Lane 1 : Whole cell lysate from HeLa cells treated with Hydroxyurea
Lane 2 : Whole cell lysate from HeLa cells mock treated
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabbit IgG
Predicted band size: 34 kDaChemiluminescence with an exposure time of 10 seconds.
Lysates prepared using NETN lysis buffer.
Lower panel shows western blot for total CDK1 using rabbit anti-CDK1 recombinant monoclonal.
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![Immunoprecipitation - Anti-CDK1 (phospho Y15) antibody [BLR101H] (ab275958)](https://www.abcam.com/ps/products/275/ab275958/Images/ab275958-411887-antiCDK1antibodyBLR101HimmunoprecipitationHeLacellshuman1.jpg)
Immunoprecipitation - Anti-CDK1 (phospho Y15) antibody [BLR101H] (ab275958)CDK1 was immunoprecipitated from HeLa whole cell lysate (treated with phosphatase (+) or mock treated (-)) using ab275958 at at 20 µl/mg lysate.
Total CDK1 was immunoprecipitated by a Rabbit anti-CDK1 recombinant monoclonal antibody.
For blotting ab275958 was used at 1/1000 dilution.
Chemiluminescence with an exposure time of 10 seconds.
Lower panel shows WB for total CDK1 using rabbit anti-CDK1 recombinant monoclonal.
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![Anti-CDK1 (phospho Y15) antibody [BLR101H] (ab275958)](https://www.abcam.com/ps/products/275/ab275958/Images/ab275958-3-benefits-of-recombinant-antibodies.png)
Anti-CDK1 (phospho Y15) antibody [BLR101H] (ab275958)