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Cancer Cell cycle Kinases/phosphatases Cdks

Recombinant human CDK7 + Cyclin H + MNAT1 protein (Tagged) (ab271463)

Recombinant human CDK7 + Cyclin H + MNAT1 protein (Tagged) (ab271463)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: >= 60% SDS-PAGE
  • Active: Yes
  • Tags: His tag N-Terminus, His tag C-Terminus, His tag N-Terminus
  • Suitable for: SDS-PAGE, Functional Studies

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Description

  • Product name

    Recombinant human CDK7 + Cyclin H + MNAT1 protein (Tagged)
    See all CDK7 + Cyclin H + MNAT1 proteins and peptides
  • Biological activity

    Specific Activity: 21 pmol/min/µg
     
    Inhibition of CDK7/Cyclin H/MAT1 enzyme by Dinaciclib, measured using a CD7 assay kit.
  • Purity

    >= 60 % SDS-PAGE.

  • Expression system

    Baculovirus infected Sf9 cells
  • Accession

    P50613
    P51946
    P51948
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Amino Acid Sequence 1

    • Species

      Human
    • Sequence

      MALDVKSRAK RYEKLDFLGE GQFATVYKAR DKNTNQIVAI KKIKLGHRSE AKDGINRTAL REIKLLQELS HPNIIGLLDA FGHKSNISLV FDFMETDLEV IIKDNSLVLT PSHIKAYMLM TLQGLEYLHQ HWILHRDLKP NNLLLDENGV LKLADFGLAK SFGSPNRAYT HQVVTRWYRA PELLFGARMY GVGVDMWAVG CILAELLLRV PFLPGDSDLD QLTRIFETLG TPTEEQWPDM CSLPDYVTFK SFPGIPLHHI FSAAGDDLLD LIQGLFLFNP CARITATQAL KMKYFSNRPG PTPGCQLPRP NCPVETLKEQ SNPALAIKRK RTEALEQGGL PKKLIF
    • Predicted molecular weight

      40 kDa
    • Amino acids

      2 to 346
    • Tags

      His tag N-Terminus
    • Additional sequence information

      CDK7
    • Amino Acid Sequence 2

    • Species

      Human
    • Sequence

      MYHNSSQKRH WTFSSEEQLA RLRADANRKF RCKAVANGKV LPNDPVFLEP HEEMTLCKYY EKRLLEFCSV FKPAMPRSVV GTACMYFKRF YLNNSVMEYH PRIIMLTCAF LACKVDEFNV SSPQFVGNLR ESPLGQEKAL EQILEYELLL IQQLNFHLIV HNPYRPFEGF LIDLKTRYPI LENPEILRKT ADDFLNRIAL TDAYLLYTPS QIALTAILSS ASRAGITMES YLSESLMLKE NRTCLSQLLD IMKSMRNLVK KYEPPRSEEV AVLKQKLERC HSAELALNVI TKKRKGYEDD DYVSKKSKHE EEEWTDDDLV ESL
    • Amino acids

      1 to 323
    • Tags

      His tag C-Terminus
    • Additional sequence information

      Cyclin H
    • Amino Acid Sequence 3

    • Species

      Human
    • Sequence

      MDDQGCPRCK TTKYRNPSLK LMVNVCGHTL CESCVDLLFV RGAGNCPECG TPLRKSNFRV QLFEDPTVDK EVEIRKKVLK IYNKREEDFP SLREYNDFLE EVEEIVFNLT NNVDLDNTKK KMEIYQKENK DVIQKNKLKL TREQEELEEA LEVERQENEQ RRLFIQKEEQ LQQILKRKNK QAFLDELESS DLPVALLLAQ HKDRSTQLEM QLEKPKPVKP VTFSTGIKMG QHISLAPIHK LEEALYEYQP LQIETYGPHV PELEMLGRLG YLNHVRAASP QDLAGGYTSS LACHRALQDA FSGLFWQPS
    • Amino acids

      2 to 309
    • Tags

      His tag N-Terminus
    • Additional sequence information

      MNAT1

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 7
    Preservative: 1.54% Imidazole
    Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine)

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

Images

  • SDS-PAGE - Recombinant Human CDK7 + Cyclin H + MNAT1 protein (Tagged) (ab271463)
    SDS-PAGE - Recombinant Human CDK7 + Cyclin H + MNAT1 protein (Tagged) (ab271463)
    SDS-PAGE analysis of ab271463.
  • Functional Studies - Recombinant Human CDK7 + Cyclin H + MNAT1 protein (Tagged) (ab271463)
    Functional Studies - Recombinant Human CDK7 + Cyclin H + MNAT1 protein (Tagged) (ab271463)

    Specific activity of ab271463.

    Inhibition of CDK7/Cyclin H/MAT1 enzyme by Dinaciclib, measured using a CD7 assay kit.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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